铝会干扰激素刺激的肝细胞中振荡性磷酸肌醇介导的钙信号传导。
Aluminium perturbs oscillatory phosphoinositide-mediated calcium signalling in hormone-stimulated hepatocytes.
作者信息
Schöfl C, Sanchez-Bueno A, Dixon C J, Woods N M, Lee J A, Cuthbertson K S, Cobbold P H, Birchall J D
机构信息
Department of Human Anatomy and Cell Biology, University of Liverpool, U.K.
出版信息
Biochem J. 1990 Jul 15;269(2):547-50. doi: 10.1042/bj2690547.
Abstract
Aluminium is known to be toxic to cells from bone, brain and bone marrow but the molecular target(s) affected by Al3+ are not known. We show here that Al3+ disrupts the oscillatory free Ca2+ responses of hepatocytes exposed to the Ca2(+)-mobilizing agonist phenylephrine. Al3+ initially increases the frequency of the oscillations and later induces broad Ca2+ spikes lasting several minutes. These broad spikes persist after removal of both agonist and Al3+ from the medium. In the absence of agonist, Al3+ has no effect on free Ca2+. The data suggest that some component(s) of the receptor-phosphoinositide-Ca2+ signalling pathway might be the site at which Al3+ exerts toxic effects.
摘要
已知铝对来自骨骼、大脑和骨髓的细胞具有毒性，但尚不清楚受Al3+影响的分子靶点。我们在此表明，Al3+会破坏暴露于钙离子动员激动剂去氧肾上腺素的肝细胞的振荡性游离钙离子反应。Al3+最初会增加振荡频率，随后诱导持续数分钟的广泛钙离子尖峰。在从培养基中去除激动剂和Al3+后，这些广泛的尖峰仍然存在。在没有激动剂的情况下，Al3+对游离钙离子没有影响。数据表明，受体-磷酸肌醇-钙离子信号通路的某些成分可能是Al3+发挥毒性作用的位点。
相似文献
1
Aluminium perturbs oscillatory phosphoinositide-mediated calcium signalling in hormone-stimulated hepatocytes.铝会干扰激素刺激的肝细胞中振荡性磷酸肌醇介导的钙信号传导。
Biochem J. 1990 Jul 15;269(2):547-50. doi: 10.1042/bj2690547.
2
Agonist-specificity in the role of Ca(2+)-induced Ca2+ release in hepatocyte Ca2+ oscillations.肝细胞钙离子振荡中钙诱导钙释放作用的激动剂特异性
Biochem J. 1993 Apr 1;291 ( Pt 1)(Pt 1):169-72. doi: 10.1042/bj2910169.
3
Bovine growth hormone induces oscillations in cytosolic free Ca2+ in single rat hepatocytes.牛生长激素可诱导单个大鼠肝细胞胞质游离钙离子产生振荡。
Biochem J. 1996 Jan 15;313 ( Pt 2)(Pt 2):525-8. doi: 10.1042/bj3130525.
4
Agonist-specific behaviour of the intracellular Ca2+ response in rat hepatocytes.大鼠肝细胞内钙离子反应的激动剂特异性行为
Biochem J. 1997 Dec 1;328 ( Pt 2)(Pt 2):573-9. doi: 10.1042/bj3280573.
5
Elevated intracellular cyclic AMP exerts different modulatory effects on cytosolic free Ca2+ oscillations induced by ADP and ATP in single rat hepatocytes.细胞内环状AMP水平升高对单个大鼠肝细胞中由ADP和ATP诱导的胞质游离Ca2+振荡产生不同的调节作用。
Biochem J. 1994 Sep 15;302 ( Pt 3)(Pt 3):949-55. doi: 10.1042/bj3020949.
6
Calcium and the endothelin-1 and alpha 1-adrenergic stimulated phosphatidylinositol cycle in cultured rat cardiomyocytes.钙与培养的大鼠心肌细胞中内皮素-1和α1-肾上腺素能刺激的磷脂酰肌醇循环
J Mol Cell Cardiol. 1994 Aug;26(8):1081-93. doi: 10.1006/jmcc.1994.1128.
7
Thimerosal enhances agonist-specific differences between [Ca2+]i oscillations induced by phenylephrine and ATP in single rat hepatocytes.硫柳汞增强了苯肾上腺素和三磷酸腺苷在单个大鼠肝细胞中诱导的[Ca2+]i振荡之间的激动剂特异性差异。
Cell Calcium. 1999 Feb;25(2):173-8. doi: 10.1054/ceca.1998.0017.
8
Modulation of cytosolic-[Ca2+] oscillations in hepatocytes results from cross-talk among second messengers. The synergism between the alpha 1-adrenergic response, glucagon and cyclic AMP, and their antagonism by insulin and diacylglycerol manifest themselves in the control of the cytosolic-[Ca2+] oscillations.肝细胞中胞质[Ca2+]振荡的调节源于第二信使之间的相互作用。α1-肾上腺素能反应、胰高血糖素和环磷酸腺苷之间的协同作用，以及它们与胰岛素和二酰基甘油之间的拮抗作用，在胞质[Ca2+]振荡的控制中得以体现。
Biochem J. 1992 Sep 15;286 ( Pt 3)(Pt 3):869-77. doi: 10.1042/bj2860869.
9
Anti-diabetic biguanides inhibit hormone-induced intracellular Ca2+ concentration oscillations in rat hepatocytes.抗糖尿病双胍类药物可抑制激素诱导的大鼠肝细胞内钙离子浓度振荡。
Biochem J. 1994 Dec 1;304 ( Pt 2)(Pt 2):561-7. doi: 10.1042/bj3040561.
10
Epidermal growth factor induces dose-dependent calcium oscillations in single fura-2-loaded hepatocytes.
Hepatology. 1992 Aug;16(2):479-86. doi: 10.1002/hep.1840160229.
引用本文的文献
1
Aluminium impacts elements of the phosphoinositide signalling pathway in neuroblastoma cells.铝影响神经母细胞瘤细胞中磷酸肌醇信号通路的元件。
Mol Cell Biochem. 1993 Apr 21;121(2):109-18. doi: 10.1007/BF00925969.
2
Aluminum interaction with phosphoinositide-associated signal transduction.铝与磷酸肌醇相关信号转导的相互作用。
Arch Toxicol. 1994;68(1):1-7. doi: 10.1007/s002040050023.
3
Intestinal perfusion of dietary levels of aluminium: association with the mucosa.饮食水平铝的肠道灌注：与黏膜的关联
Gut. 1994 Aug;35(8):1053-7. doi: 10.1136/gut.35.8.1053.
4
Possible factors in the etiology of Alzheimer's disease.阿尔茨海默病病因中的可能因素。
Mol Neurobiol. 1994 Aug-Dec;9(1-3):1-13. doi: 10.1007/BF02816099.
5
Effects of aluminium on the hepatic inositol polyphosphate phosphatase.铝对肝脏肌醇多磷酸磷酸酶的影响。
Biochem J. 1995 Jan 15;305 ( Pt 2)(Pt 2):557-61. doi: 10.1042/bj3050557.
6
The perturbation, by aluminium, of receptor-generated calcium transients in hepatocytes is not due to effects of Ins(1,4,5)P3-stimulated Ca2+ release or Ins(1,4,5)P3 metabolism by the 5-phosphatase and 3-kinase.铝对肝细胞中受体产生的钙瞬变的干扰并非由于Ins(1,4,5)P3刺激的Ca2+释放或5-磷酸酶和3-激酶对Ins(1,4,5)P3代谢的影响。
Biochem J. 1990 Sep 15;270(3):837. doi: 10.1042/bj2700837a.
7
Electroporation can cause artefacts due to solubilization of cations from the electrode plates. Aluminum ions enhance conversion of inositol 1,3,4,5-tetrakisphosphate into inositol 1,4,5-trisphosphate in electroporated L1210 cells.电穿孔可能会因电极板上阳离子的溶解而导致假象。铝离子可增强电穿孔处理的L1210细胞中肌醇1,3,4,5-四磷酸向肌醇1,4,5-三磷酸的转化。
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):883-5. doi: 10.1042/bj2770883.
8
Aluminum effect on the activity of superoxide dismutase and of other antioxygenic enzymes in vitro.铝对超氧化物歧化酶及其他抗氧化酶体外活性的影响。
Biol Trace Elem Res. 1991 Oct;31(1):79-96. doi: 10.1007/BF02990362.
本文引用的文献
1
THE BIOCHEMISTRY OF DESFERRIOXAMINE AND ITS RELATION TO IRON METABOLISM.去铁胺的生物化学及其与铁代谢的关系。
Ann N Y Acad Sci. 1964 Oct 7;119:758-68. doi: 10.1111/j.1749-6632.1965.tb54077.x.
2
The dialysis dementia syndrome and aluminum intoxication.
Nephron. 1982;31(1):1-10. doi: 10.1159/000182595.
3
Therapy of aluminium overload (II).
Contrib Nephrol. 1984;38:78-80. doi: 10.1159/000408069.
4
Aluminium poisoning: dialysis encephalopathy, osteomalacia, and anaemia.铝中毒：透析性脑病、骨软化症和贫血。
Lancet. 1983 Jul 2;2(8340):29-34. doi: 10.1016/s0140-6736(83)90014-4.
5
Comparison of metal-binding properties of trans-1,2-cyclohexanediol diphosphate and deacylated phosphoinositides.反式-1,2-环己二醇二磷酸酯与脱酰基磷酸肌醇的金属结合特性比较。
Biochemistry. 1969 Dec;8(12):4855-8. doi: 10.1021/bi00840a031.
6
Relationship of aluminum to Alzheimer's disease.铝与阿尔茨海默病的关系。
Environ Health Perspect. 1985 Nov;63:149-53. doi: 10.1289/ehp.8563149.
7
Repetitive transient rises in cytoplasmic free calcium in hormone-stimulated hepatocytes.激素刺激的肝细胞中细胞质游离钙的重复性短暂升高。
Nature. 1986;319(6054):600-2. doi: 10.1038/319600a0.
8
Aluminum in acidic surface waters: chemistry, transport, and effects.酸性地表水中的铝：化学性质、迁移及影响
Environ Health Perspect. 1985 Nov;63:93-104. doi: 10.1289/ehp.856393.
9
Agonist-induced oscillations in cytoplasmic free calcium concentration in single rat hepatocytes.激动剂诱导的单个大鼠肝细胞胞质游离钙浓度振荡
Cell Calcium. 1987 Feb;8(1):79-100. doi: 10.1016/0143-4160(87)90038-8.
10
Aluminum ion in biological systems.生物系统中的铝离子。
Trends Biochem Sci. 1988 Jan;13(1):15-9. doi: 10.1016/0968-0004(88)90012-6.
Zotero 插件