Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, Cape Town, South Africa.
Antonie Van Leeuwenhoek. 2011 Nov;100(4):483-96. doi: 10.1007/s10482-011-9604-z. Epub 2011 Jun 14.
Partial recN gene sequences (>1 kb) were obtained from 35 type strains of the genus Amycolatopsis. Phylogenetic trees were constructed to determine the effectiveness of using this gene to predict taxonomic relationships within the genus. The use of recN sequence analysis as an alternative to DNA-DNA hybridization (DDH) for distinguishing closely related species was also assessed. The recN based phylogeny mostly confirmed the conventional 16S rRNA and gyrB gene-based phylogenies and thus provides further support for these phylogenetic groupings. As is the case for the gyrB gene, pairwise recN sequence similarities cannot be used to predict the DNA relatedness between type strains but the recN genetic distance can be used as a means to assess quickly whether an isolate is likely to represent a new species in the genus Amycolatopsis. A recN genetic distance of >0.04 between two Amycolatopsis strains is proposed to provide a good indication that they belong to different species (and that polyphasic taxonomic characterization of the unknown strain is worth undertaking).
从 35 株链霉菌属的模式菌株中获得了部分 recN 基因序列（>1 kb）。构建系统发育树以确定该基因在预测属内分类关系方面的有效性。还评估了使用 recN 序列分析作为区分密切相关物种的 DNA-DNA 杂交（DDH）替代方法的有效性。recN 系统发育树主要证实了传统的 16S rRNA 和 gyrB 基因系统发育树，因此为这些系统发育分组提供了进一步的支持。与 gyrB 基因一样，不能使用成对的 recN 序列相似性来预测模式菌株之间的 DNA 相关性，但 recN 遗传距离可用于快速评估分离株是否可能代表链霉菌属中的一个新物种。建议 recN 遗传距离>0.04 可作为两个链霉菌菌株属于不同物种的良好指标（并且值得对未知菌株进行多相分类特征描述）。
