Blednov Iu A, Gordeĭ M L, Seredenin S B
Biull Eksp Biol Med. 1990 Apr;109(4):385-7.
The displacement curves of the effect of GABA on the S35-tert-butyl bicyclophosphorothionate binding to the brain membranes of inbred mice were analysed. It was revealed the lack of marked interstrain differences in dependent on the ionic force of incubation medium modification of IC50. After incomplete stimulation of Cl(-)-ionophore subunit (50 mM NaCl) reliable interstrain differences in the value of nHill were shown. Bicuculline in the same conditions prevented the inhibitory effect of GABA (10(-5) M). But submaximal concentrations of bicuculline (5 X 10(-6) M, 10(-6) M) stimulated the radioligand binding in the presence of GABA (10(-6) M). It was marked more expressed bicuculline stimulation effect on the membranes of C57Bl/6 mice, as compared to BALB/c mice.
分析了γ-氨基丁酸（GABA）对近交系小鼠脑膜中S35-叔丁基双环磷硫代酸盐结合作用的位移曲线。结果显示，在依赖于孵育介质离子强度对半数抑制浓度（IC50）进行修饰时，缺乏明显的品系间差异。在对氯离子载体亚基进行不完全刺激（50 mM氯化钠）后，显示出Hill系数（nHill）值存在可靠的品系间差异。在相同条件下，荷包牡丹碱可阻止GABA（10^(-5) M）的抑制作用。但亚最大浓度的荷包牡丹碱（5×10^(-6) M、10^(-6) M）在GABA（10^(-6) M）存在的情况下刺激放射性配体结合。与BALB/c小鼠相比，在C57Bl/6小鼠的脑膜上，荷包牡丹碱的刺激作用更为明显。
