Blednov Iu A, Gordeĭ M L, Seredenin S B
Biull Eksp Biol Med. 1989 Nov;108(11):567-9.
The displacement curves of the effect of picrotoxin on the S35-tert-butyl bicyclophosphorothionate binding to the brain membranes of inbred mice C57Bl/6 and Balb/c were analysed. The marked interstrain differences between modifications of IC50 and nHill dependent on the ionic force of the incubation medium were revealed. After partial stimulation of the receptor in the presence of 50 mM concentration of NaCl, the susceptibility of the receptor to picrotoxin was higher in the membrane suspension of C57Bl/6 mice than of Balb/c. Full stimulation of the receptor by 200 mM concentration of NaCl resulted in disappearance of interstrain differences. The value of nHill of C57Bl/6 membrane receptors increased after the change of the concentration of NaCl in the incubation medium from 50 to 200 mM, while it was invariable to Balb/c mice. It was marked that modification of the parameters of the binding of the radioligand to the membranes of C57Bl/6 mice was more latent, than for Balb/c mice.
分析了印防己毒素对S35-叔丁基双环硫代磷酸酯与近交系C57Bl/6和Balb/c小鼠脑膜结合作用的位移曲线。发现取决于孵育介质离子强度的IC50和nHill修饰存在明显的品系间差异。在50 mM浓度的NaCl存在下对受体进行部分刺激后，C57Bl/6小鼠膜悬液中受体对印防己毒素的敏感性高于Balb/c小鼠。200 mM浓度的NaCl对受体进行完全刺激导致品系间差异消失。当孵育介质中NaCl浓度从50 mM变为200 mM时，C57Bl/6膜受体的nHill值增加，而对Balb/c小鼠则不变。值得注意的是，与Balb/c小鼠相比，放射性配体与C57Bl/6小鼠脑膜结合参数的修饰更不明显。
