Guarner J, del Rio C, Hendrix L, Unger E R
Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322.
Cancer. 1990 Aug 15;66(4):796-800. doi: 10.1002/1097-0142(19900815)66:4<796::aid-cncr2820660433>3.0.co;2-u.
A 44-year-old man infected with human immunodeficiency virus had Hodgkin's disease, mixed cellularity, and malignant non-Hodgkin's lymphoma, diffuse large cell type. Colorimetric in-situ hybridization showed the Epstein-Barr virus (EBV) genome in the cells of the large cell non-Hodgkin's lymphoma and in the Reed-Sternberg cells and reactive lymphocytes of the Hodgkin's lymphoma. These results suggest that EBV may play a similar causative role in both neoplasms. This colorimetric method of hybridization, yielding results within 8 hours, is applicable to archival material and will be useful in further epidemiologic work associating EBV and lymphoid proliferations and malignancies.
一名感染人类免疫缺陷病毒的44岁男性患有混合细胞型霍奇金病和弥漫大细胞型恶性非霍奇金淋巴瘤。比色原位杂交显示，在大细胞非霍奇金淋巴瘤细胞以及霍奇金淋巴瘤的里德-斯腾伯格细胞和反应性淋巴细胞中存在爱泼斯坦-巴尔病毒（EBV）基因组。这些结果表明，EBV可能在这两种肿瘤中发挥类似的致病作用。这种比色杂交方法在8小时内即可得出结果，适用于存档材料，将有助于进一步开展将EBV与淋巴组织增生及恶性肿瘤相关联的流行病学研究。
