Ronai Z A, Weinstein I B
Comprehensive Cancer Center, Columbia University, New York, New York 10032.
Cancer Res. 1990 Sep 1;50(17):5374-81.
The exposure of mammalian cells to UV light or other DNA-damaging agents induces several responses which may provide cellular defense mechanisms and also play a role in carcinogenesis. Employing a 257-base pair DNA fragment from the polyoma virus that contains the origin of replication and regulatory region of this virus, we have identified a set of DNA-binding proteins that are induced in normal rat fibroblasts at 6-24 h after UV exposure. These proteins bind to a specific octamer sequence (TGACAACA) designated the "UV response element." Purification of these inducible proteins on a UV response element affinity column revealed a set of proteins, among which the major protein has a molecular weight of 40,000, which co-purify with c-fos but do not react with antibodies to c-jun-encoded proteins. These UV-induced proteins may, in concert with other cellular components, play a role in mediating specific cellular responses to DNA damage in mammalian cells.
哺乳动物细胞暴露于紫外线或其他DNA损伤剂会引发多种反应，这些反应可能提供细胞防御机制，并且在致癌过程中也发挥作用。利用来自多瘤病毒的一段257个碱基对的DNA片段，该片段包含该病毒的复制起点和调控区域，我们鉴定出一组DNA结合蛋白，它们在紫外线照射后6至24小时在正常大鼠成纤维细胞中被诱导产生。这些蛋白与一个特定的八聚体序列（TGACAACA）结合，该序列被称为“紫外线反应元件”。在紫外线反应元件亲和柱上对这些可诱导蛋白进行纯化，揭示出一组蛋白，其中主要蛋白的分子量为40,000，它与c-fos共同纯化，但不与针对c-jun编码蛋白的抗体发生反应。这些紫外线诱导的蛋白可能与其他细胞成分协同作用，在介导哺乳动物细胞对DNA损伤的特定细胞反应中发挥作用。
