Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Poult Sci. 2011 Jul;90(7):1555-61. doi: 10.3382/ps.2010-01333.
Animals may suffer from a variety of environmental stressors every day, among which is cold stress, which commonly exists in cold regions. The aim of this study was to investigate changes in antioxidative function and inducible nitric oxide synthase-nitric oxide (iNOS-NO) system activity in the duodenum of chicks as a reaction to cold stress. A total of 84 male chicks (15 d old) were randomly allocated to 12 groups (7 chickens/group). There were 1 control group and 5 treatment groups for acute cold stress and 3 control groups and 3 treatment groups for chronic cold stress. Antioxidative function was examined by superoxide dismutase (SOD), and oxidative damage was examined by malondialdehyde (MDA) detection. The iNOS-NO system activity was identified by NO content and NOS activity assay, and the transcription of iNOS mRNA was tested by fluorescence quantitative PCR. The results showed that under acute cold stress MDA level increased, the activity of NO in the duodenum fluctuated, and the activity of SOD and iNOS in the duodenum first increased and then decreased, whereas the expression of iNOS mRNA decreased. Under chronic cold stress the activity of SOD, NO, and NOS first decreased and then increased, whereas the MDA level and the expression of iNOS mRNA increased. The results indicated that both acute and chronic cold stress could cause duodenum oxidative stress and change in iNOS, which was related to the intestinal damage process.
动物每天可能会遭受各种环境应激源的影响，其中包括冷应激，这种应激源在寒冷地区普遍存在。本研究旨在探讨冷应激对雏鸡十二指肠抗氧化功能和诱导型一氧化氮合酶-一氧化氮（iNOS-NO）系统活性的变化。将 84 只雄性雏鸡（15 日龄）随机分配到 12 个组（每组 7 只鸡）。其中有 1 个对照组和 5 个急性冷应激处理组，以及 3 个对照组和 3 个慢性冷应激处理组。通过超氧化物歧化酶（SOD）检测抗氧化功能，通过丙二醛（MDA）检测评估氧化损伤。通过 NO 含量和 NOS 活性测定来确定 iNOS-NO 系统活性，并通过荧光定量 PCR 检测 iNOS mRNA 的转录。结果表明，在急性冷应激下 MDA 水平升高，十二指肠中 NO 活性波动，十二指肠中 SOD 和 iNOS 活性先升高后降低，而 iNOS mRNA 的表达降低。在慢性冷应激下，SOD、NO 和 NOS 的活性先降低后升高，而 MDA 水平和 iNOS mRNA 的表达增加。结果表明，急性和慢性冷应激均可导致十二指肠氧化应激和 iNOS 改变，这与肠道损伤过程有关。
