Aviñó Anna, Grijalvo Santiago, Pérez-Rentero Sónia, Garibotti Alejandra, Terrazas Montserrat, Eritja Ramon
Methods Mol Biol. 2011;751:223-38. doi: 10.1007/978-1-61779-151-2_14.
Oligonucleotide-peptide conjugates have attracted considerable interest especially for biomedical uses. In the first part of this chapter, we describe protocols for the stepwise synthesis of oligonucleotides carrying peptide sequences at the 3'-end on a single support. The resulting oligonucleotide-peptide conjugates may be used as exogenous effectors for the specific control of gene expression. In the second part of this chapter, detailed postsynthetic conjugation protocols to introduce peptide sequences into oligonucleotide sequences are also presented.
寡核苷酸 - 肽缀合物尤其在生物医学应用方面引起了广泛关注。在本章的第一部分，我们描述了在单个载体上逐步合成3'端带有肽序列的寡核苷酸的方案。所得的寡核苷酸 - 肽缀合物可用作基因表达特异性控制的外源性效应物。在本章的第二部分，还介绍了将肽序列引入寡核苷酸序列的详细合成后缀合方案。
