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寡核苷酸-肽缀合物：在酸性条件下的固相合成及其在 ELISA 测定中的应用。

Oligonucleotide-peptide conjugates: solid-phase synthesis under acidic conditions and use in ELISA assays.

机构信息

Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Jordi Girona 18, E-08034 Barcelona, Spain.

出版信息

Molecules. 2012 Nov 22;17(12):13825-43. doi: 10.3390/molecules171213825.

DOI:10.3390/molecules171213825
PMID:23174899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6268656/
Abstract

Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients.

摘要

在这里，我们使用固相方法制备携带纤维蛋白/角蛋白丝氨酸化肽的寡核苷酸。 合成后缀合方案成功应用于携带小肽的寡核苷酸的合成。 使用酸处理进行最终脱保护的逐步方案允许制备携带更长和富含精氨酸的肽的嘧啶寡核苷酸。 开发了基于 ELISA 的测试，使用寡核苷酸-丝氨酸化肽缀合物检测类风湿关节炎患者血清中的抗丝氨酸化蛋白/肽抗体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/0f75063f3d46/molecules-17-13825-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/991c006908f9/molecules-17-13825-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/061e9cd4d6c0/molecules-17-13825-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/1e17184c03fe/molecules-17-13825-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/5ed3f55a2dfd/molecules-17-13825-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/8ca2a61e18b3/molecules-17-13825-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/7c5b85a3b0fa/molecules-17-13825-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/206180ca6843/molecules-17-13825-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/ef146f50c18f/molecules-17-13825-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/0f75063f3d46/molecules-17-13825-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/991c006908f9/molecules-17-13825-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/061e9cd4d6c0/molecules-17-13825-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/1e17184c03fe/molecules-17-13825-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/5ed3f55a2dfd/molecules-17-13825-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/8ca2a61e18b3/molecules-17-13825-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/7c5b85a3b0fa/molecules-17-13825-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/206180ca6843/molecules-17-13825-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/ef146f50c18f/molecules-17-13825-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30f/6268656/0f75063f3d46/molecules-17-13825-g009.jpg

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本文引用的文献

1
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Chem Commun (Camb). 2012 Jun 18;48(48):5992-4. doi: 10.1039/c2cc32010j. Epub 2012 May 10.
2
The use of chimeric vimentin citrullinated peptides for the diagnosis of rheumatoid arthritis.用嵌合波形蛋白瓜氨酸化肽进行类风湿关节炎的诊断。
J Med Chem. 2011 Nov 10;54(21):7486-92. doi: 10.1021/jm200563u. Epub 2011 Oct 7.
3
An activated triple bond linker enables 'click' attachment of peptides to oligonucleotides on solid support.
Molecules. 2021 Sep 6;26(17):5420. doi: 10.3390/molecules26175420.
4
Meta-analysis: diagnostic accuracy of the citrullinated peptides derived from fibrinogen and vimentin in rheumatoid arthritis.Meta 分析：纤维蛋白原和波形蛋白衍生的瓜氨酸化肽在类风湿关节炎中的诊断准确性。
Clin Rheumatol. 2020 Apr;39(4):1111-1120. doi: 10.1007/s10067-019-04845-2. Epub 2019 Dec 19.
一个活化的三键连接子使得在固体载体上的寡核苷酸能够通过点击反应连接到肽上。
Nucleic Acids Res. 2011 Nov 1;39(20):9047-59. doi: 10.1093/nar/gkr603. Epub 2011 Jul 27.
4
Multiplexed binding determination of seven glycoconjugates for Pseudomonas aeruginosa lectin I (PA-IL) using a DNA-based carbohydrate microarray.基于 DNA 的糖芯片技术对铜绿假单胞菌凝集素 I（PA-IL）与七种糖缀合物的复配结合测定。
Chem Commun (Camb). 2011 Aug 21;47(31):8826-8. doi: 10.1039/c1cc12428e. Epub 2011 Jul 11.
5
Synthesis of oligonucleotide-peptide conjugates for biomedical and technological applications.用于生物医学和技术应用的寡核苷酸 - 肽缀合物的合成。
Methods Mol Biol. 2011;751:223-38. doi: 10.1007/978-1-61779-151-2_14.
6
Stepwise synthesis of oligonucleotide-peptide conjugates containing guanidinium and lipophilic groups in their 3'-termini.3’-末端含胍基和脂环族基团的寡核苷酸-肽缀合物的逐步合成。
Bioorg Med Chem Lett. 2010 Apr 1;20(7):2144-7. doi: 10.1016/j.bmcl.2010.02.049. Epub 2010 Feb 14.
7
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Cell Mol Life Sci. 2010 Mar;67(5):715-26. doi: 10.1007/s00018-009-0186-0. Epub 2009 Nov 7.
8
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9
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Arthritis Res Ther. 2009;11(5):R135. doi: 10.1186/ar2802. Epub 2009 Sep 2.