受化学刺激调控的鸟嘌呤四联体、G-四链体和G-线

G-quartet, G-quadruplex, and G-wire regulated by chemical stimuli.

作者信息

Miyoshi Daisuke, Sugimoto Naoki

机构信息

Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), and Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, Kobe, Japan.

出版信息

Methods Mol Biol. 2011;749:93-104. doi: 10.1007/978-1-61779-142-0_7.

DOI:10.1007/978-1-61779-142-0_7

PMID:21674367

Abstract

Guanine-rich DNA, which is widely distributed in the human genome, can fold into a supramolecular structure called the G-wire. The G-wire possesses promising characteristics as a functional element for various applications in vitro and in vivo. Here, we describe the preparative procedures for the G-wire and signatures of G-wire formation. Procedures for the regulation of G-wire formation by chemical stimuli will be useful for in vivo and in vitro applications.

摘要

富含鸟嘌呤的DNA广泛分布于人类基因组中，能够折叠成一种称为G-线的超分子结构。G-线作为一种功能性元件，在体外和体内的各种应用中具有良好的特性。在此，我们描述了G-线的制备程序以及G-线形成的特征。通过化学刺激调节G-线形成的程序将对体内和体外应用有用。