可视化 DNA 纳米结构中 G-四链体的动态构象转变。
Visualization of dynamic conformational switching of the G-quadruplex in a DNA nanostructure.
机构信息
Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
出版信息
J Am Chem Soc. 2010 Nov 24;132(46):16311-3. doi: 10.1021/ja1058907. Epub 2010 Oct 28.
We herein report the real-time observation of G-quadruplex formation by monitoring the G-quadruplex-induced global change of two duplexes incorporated in a DNA nanoscaffold. The introduced G-rich strands formed an interstrand (3 + 1) G-quadruplex structure in the presence of K(+), and the formed four-stranded structure was disrupted by removal of K(+). These conformational changes were visualized in a nanoscaffold in real-time with fast-scanning atomic force microscopy.
我们在此通过监测结合在 DNA 纳米支架中的两条双链体的 G-四链体诱导的整体变化，实时观察 G-四链体的形成。在存在 K(+)的情况下，引入的富含 G 的链形成了链间（3 + 1）G-四链体结构，并且形成的四链体结构通过除去 K(+)而被破坏。这些构象变化可以通过快速扫描原子力显微镜在纳米支架中实时可视化。
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