Department of Medical Microbiology, University Medical Center Groningen and University of Groningen, Hanzeplein 1, Groningen, The Netherlands.
Proteomics. 2011 Jul;11(14):2921-30. doi: 10.1002/pmic.201100134. Epub 2011 Jun 14.
The human commensal bacterium Staphylococcus aureus is renowned as a causative agent of severe invasive diseases. Upon entering the bloodstream, S. aureus can infect almost every tissue and organ system in the human body. To withstand insults from the immune system upon invasion, several immune-evasive mechanisms have evolved in S. aureus, such as complement inhibition by secreted proteins and IgG-binding by surface-exposed protein A. While it is generally accepted that S. aureus cells bind a range of host factors for various purposes, no global analyses to profile staphylococcal host factor binding have so far been performed. Therefore, we explored the possibility to profile the binding of human serum proteins to S. aureus cells by "surface shaving" with trypsin and subsequent MS analysis of liberated peptides. This resulted in the identification of several components of the complement system, the platelet factor 4 and the isoform 1 of the inter-α-trypsin inhibitor heavy chain H4 on the staphylococcal cell surface. We conclude that surface shaving is a versatile tool to profile global interactions between human serum proteins and the S. aureus cell surface.
人体共生菌金黄色葡萄球菌是一种严重侵袭性疾病的病原体。金黄色葡萄球菌进入血液后，几乎可以感染人体的所有组织和器官系统。为了在入侵时抵御免疫系统的攻击，金黄色葡萄球菌进化出了几种免疫逃避机制，例如分泌蛋白对补体的抑制作用和表面暴露的蛋白 A 对 IgG 的结合作用。虽然人们普遍认为金黄色葡萄球菌细胞会结合一系列宿主因子来达到不同的目的，但迄今为止尚未进行全面分析来描述金黄色葡萄球菌对宿主因子的结合情况。因此，我们通过使用胰蛋白酶进行“表面刮削”，并随后对释放的肽进行 MS 分析，探索了描述人类血清蛋白与金黄色葡萄球菌细胞结合的可能性。这导致鉴定了补体系统的几个成分、血小板因子 4 和α-胰蛋白酶抑制剂重链 H4 的同工型 1 位于金黄色葡萄球菌细胞表面。我们的结论是，表面刮削是一种通用的工具，可以全面描述人类血清蛋白与金黄色葡萄球菌细胞表面之间的相互作用。
