INRAE, UCA, UMR0454 MEDIS, 63000 Clermont-Ferrand, France.
GSK, 53100 Siena, Italy.
Cells. 2023 Jun 15;12(12):1634. doi: 10.3390/cells12121634.
is a versatile commensal species of the animal gut that can also be a pathogen able to cause intestinal and extraintestinal infections. The plasticity of its genome has led to the evolution of pathogenic strains, which represent a threat to global health. Additionally, strains are major drivers of antibiotic resistance, highlighting the urgent need for new treatment and prevention measures. The antigenic and structural heterogeneity of enterohaemorrhagic colonisation factors has limited their use for the development of effective and cross-protective vaccines. However, the emergence of new strains that express virulence factors deriving from different diarrhoeagenic pathotypes suggests that a vaccine targeting conserved proteins could be a more effective approach. In this study, we conducted proteomics analysis and functional protein characterisation to identify a group of proteins potentially involved in the adhesion of O157:H7 to the extracellular matrix and intestinal epithelial cells. Among them, OmpA has been identified as a highly conserved and immunogenic antigen, playing a significant role in the adhesion phenotype of O157:H7 and in bacterial aggregation. Furthermore, antibodies raised against recombinant OmpA effectively reduced the adhesion of O157:H7 to intestinal epithelial cells. The present work highlights the role of OmpA as a potent antigen for the development of a vaccine against intestinal pathogenic .
是一种广泛存在于动物肠道内的共生菌，但也能成为病原体，引发肠道和肠道外感染。其基因组的可塑性导致了致病菌株的进化，对全球健康构成了威胁。此外，这些菌株是抗生素耐药性的主要驱动因素，凸显了迫切需要新的治疗和预防措施。肠出血性 定植因子的抗原和结构异质性限制了它们在开发有效和交叉保护疫苗方面的应用。然而，表达来自不同 腹泻病原体类型的毒力因子的新菌株的出现表明，针对保守蛋白的疫苗可能是一种更有效的方法。在这项研究中，我们进行了蛋白质组学分析和功能蛋白特性分析，以鉴定一组可能参与 O157:H7 与细胞外基质和肠上皮细胞粘附的蛋白。其中，OmpA 被鉴定为一种高度保守和免疫原性抗原，在 O157:H7 的粘附表型和细菌聚集中发挥重要作用。此外，针对重组 OmpA 产生的抗体有效降低了 O157:H7 对肠上皮细胞的粘附。本研究强调了 OmpA 作为一种潜在的抗原在开发针对肠道致病性 的疫苗中的作用。
