Department of Ophthalmology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Curr Eye Res. 2011 Aug;36(8):699-705. doi: 10.3109/02713683.2011.571359. Epub 2011 Jun 15.
To observe the inhibitory effect of tetrandrine on haze formation after Epi-LASIK surgery in rabbits.
Twenty-seven healthy New Zealand rabbits were selected and underwent Epi-LASIK surgery. According to a self comparison principle, these rabbits were randomly divided into three groups. The first group was treated with tetrandrine eye drops (Tet), the second (negative control, NC) group was treated with pure solvent, and the third group was treated with fluorometholone (FML) solvent. Haze grades of each group were respectively observed by slit lamp exam at half-month, one month, and two months after surgery. After corneal tissues were extracted, optical microscopy, Sirius Red staining, immunohistochemistry, and semi-quantitative RT-PCR tests were conducted, in order to test collagen formation of operated eyes after surgery and expression of transformation growth factor beta 2 (TGF-β(2)) in corneal stroma.
At a half month and one month after surgery, haze grades and type III collagen expression in Tet and FML groups were significantly lower than that in NC groups (P < 0.01). No statistical difference was observed between Tet and FML groups. Immunohistochemistry showed that, at each time point after surgery, expressions of TGF-β(2) protein in Tet and FML groups were significantly lower than that in the NC group (P < 0.01), whereas there was no statistical difference between Tet and FML groups. The expression level of TGF-β(2) protein increased, reaching its peak one month after surgery. RT-PCR also showed that, at each point after surgery, the TGF-β(2) mRNA expression in Tet and FML groups was lower than that in the NC group (P < 0.01), nevertheless no statistical difference was observed between Tet and FML groups.
Like FML, Tet could inhibit haze formation in rabbits after Epi-LASIK surgery, possible through TGF-β(2)-collagen-III pathway.
观察汉防己甲素（tetrandrine）对兔准分子激光上皮下角膜磨镶术（Epi-LASIK）后混浊形成的抑制作用。
选择 27 只健康新西兰白兔，行 Epi-LASIK 手术。按自身对照原则，将兔随机分为 3 组，第 1 组用汉防己甲素眼液（Tet）滴眼，第 2 组（阴性对照组，NC）用纯溶剂滴眼，第 3 组用氟米龙（FML）溶剂滴眼。术后半个月、1 个月及 2 个月时用裂隙灯显微镜观察各组兔角膜混浊分级。术后提取角膜组织，行光学显微镜、天狼猩红染色、免疫组织化学、半定量 RT-PCR 检测，以检测术后术眼角膜胶原的形成及角膜基质中转化生长因子β2（TGF-β2）的表达。
术后半个月和 1 个月时，Tet 组和 FML 组的混浊分级和Ⅲ型胶原表达均明显低于 NC 组（P＜0.01），但 Tet 组和 FML 组间差异无统计学意义。免疫组织化学显示，术后各时间点，Tet 组和 FML 组 TGF-β2 蛋白的表达均明显低于 NC 组（P＜0.01），但 Tet 组和 FML 组间差异无统计学意义。TGF-β2 蛋白表达水平逐渐增高，术后 1 个月达高峰。RT-PCR 也显示，术后各时间点，Tet 组和 FML 组 TGF-β2 mRNA 的表达均低于 NC 组（P＜0.01），但 Tet 组和 FML 组间差异无统计学意义。
与 FML 类似，汉防己甲素也可抑制兔 Epi-LASIK 术后混浊形成，可能通过 TGF-β2-胶原-Ⅲ途径。
