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从嗜热栖热菌T2菌株中克隆α-和β-半乳糖苷酶基因及其在嗜热栖热菌HB27中的表达。

Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27.

作者信息

Koyama Y, Okamoto S, Furukawa K

机构信息

Fermentation Research Institute, AIST, MITI, Tsukuba Science City, Ibaraki, Japan.

出版信息

Appl Environ Microbiol. 1990 Jul;56(7):2251-4. doi: 10.1128/aem.56.7.2251-2254.1990.

DOI:10.1128/aem.56.7.2251-2254.1990
PMID:2167630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC184593/
Abstract

The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid of Thermus cryptic plasmid pTT8, and beta-galactosidases were expressed constitutively.

摘要

来自极端嗜热细菌嗜热栖热菌菌株T2的编码耐热α-和β-半乳糖苷酶的基因在大肠杆菌中被克隆。α-半乳糖苷酶基因位于β-半乳糖苷酶基因的下游。这些基因借助嗜热栖热菌隐蔽质粒pTT8被导入嗜热栖热菌HB27，并且β-半乳糖苷酶组成型表达。