嗜热栖热菌HB27色氨酸合成酶基因的克隆与序列分析：从影印平板培养的大肠杆菌重组菌落向感受态嗜热栖热菌细胞的质粒转移
Cloning and sequence analysis of tryptophan synthetase genes of an extreme thermophile, Thermus thermophilus HB27: plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus cells.
作者信息
Koyama Y, Furukawa K
机构信息
Fermentation Research Institute, AIST, MITI, Ibaraki, Japan.
出版信息
J Bacteriol. 1990 Jun;172(6):3490-5. doi: 10.1128/jb.172.6.3490-3495.1990.
Abstract
Tryptophan synthetase genes (trpBA) of the extreme thermophile Thermus thermophilus HB27 were cloned by a novel method of direct plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus HB27 trpB cells. The nucleotide sequences of the trpBA genes were determined. The amino acid sequences deduced from the nucleotide sequences of Thermus trpB and trpA were found to have identities of 54.8 and 28.7%, respectively, with those of E. coli trpB and trpA genes. Low cysteine content (one in trpB; zero in trpA) is a striking feature of these proteins, which may contribute to their thermostability.
摘要
嗜热栖热菌HB27的色氨酸合成酶基因（trpBA）是通过一种新方法克隆得到的，该方法是将复制品平板上的大肠杆菌重组菌落中的质粒直接转移到感受态嗜热栖热菌HB27 trpB细胞中。测定了trpBA基因的核苷酸序列。发现从嗜热栖热菌trpB和trpA的核苷酸序列推导的氨基酸序列与大肠杆菌trpB和trpA基因的氨基酸序列分别具有54.8%和28.7%的同一性。这些蛋白质的一个显著特征是半胱氨酸含量低（trpB中有一个；trpA中为零），这可能有助于它们的热稳定性。
相似文献
1
Cloning and sequence analysis of tryptophan synthetase genes of an extreme thermophile, Thermus thermophilus HB27: plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus cells.嗜热栖热菌HB27色氨酸合成酶基因的克隆与序列分析：从影印平板培养的大肠杆菌重组菌落向感受态嗜热栖热菌细胞的质粒转移
J Bacteriol. 1990 Jun;172(6):3490-5. doi: 10.1128/jb.172.6.3490-3495.1990.
2
A plasmid vector for an extreme thermophile, Thermus thermophilus.一种用于嗜热栖热菌（Thermus thermophilus）这种极端嗜热菌的质粒载体。
FEMS Microbiol Lett. 1990 Oct;60(1-2):97-101. doi: 10.1016/0378-1097(90)90352-q.
3
4
Genes and enzymes of the acetyl cycle of arginine biosynthesis in the extreme thermophilic bacterium Thermus thermophilus HB27.嗜热栖热菌HB27中精氨酸生物合成乙酰循环的基因和酶
Microbiology (Reading). 1998 Feb;144 ( Pt 2):479-492. doi: 10.1099/00221287-144-2-479.
5
Cloning and expression of the leucine gene from Thermus thermophilus in Escherichia coli.嗜热栖热菌亮氨酸基因在大肠杆菌中的克隆与表达。
Gene. 1980 Jul;10(2):137-45. doi: 10.1016/0378-1119(80)90131-6.
6
Molecular cloning and nucleotide sequence of Thermus thermophilus HB8 trpE and trpG.嗜热栖热菌HB8 trpE和trpG的分子克隆及核苷酸序列
Biochim Biophys Acta. 1988 Sep 7;950(3):303-12. doi: 10.1016/0167-4781(88)90126-1.
7
Isolation of a low-molecular-weight, multicopy plasmid, pNHK101, from Thermus sp. TK10 and its use as an expression vector for T. thermophilus HB27.从嗜热栖热菌TK10中分离出一种低分子量多拷贝质粒pNHK101，并将其用作嗜热栖热菌HB27的表达载体。
Plasmid. 2005 Jul;54(1):70-9. doi: 10.1016/j.plasmid.2004.12.004. Epub 2005 Jan 28.
8
Glutamyl-tRNA synthetase from Thermus thermophilus HB8. Molecular cloning of the gltX gene and crystallization of the overproduced protein.嗜热栖热菌HB8的谷氨酰胺-tRNA合成酶。gltX基因的分子克隆及过量表达蛋白的结晶。
Eur J Biochem. 1992 Mar 1;204(2):465-72. doi: 10.1111/j.1432-1033.1992.tb16656.x.
9
Cloning of the trp genes from the archaebacterium Methanococcus voltae: nucleotide sequence of the trpBA genes.来自古细菌沃氏甲烷球菌的色氨酸基因的克隆：trpBA基因的核苷酸序列
Mol Gen Genet. 1988 Nov;214(3):439-50. doi: 10.1007/BF00330478.
10
Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27.从嗜热栖热菌T2菌株中克隆α-和β-半乳糖苷酶基因及其在嗜热栖热菌HB27中的表达。
Appl Environ Microbiol. 1990 Jul;56(7):2251-4. doi: 10.1128/aem.56.7.2251-2254.1990.
引用本文的文献
1
Trehalose biosynthesis in Thermus thermophilus RQ-1: biochemical properties of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase.嗜热栖热菌RQ-1中海藻糖的生物合成：海藻糖-6-磷酸合酶和海藻糖-6-磷酸磷酸酶的生化特性
Extremophiles. 2005 Feb;9(1):29-36. doi: 10.1007/s00792-004-0421-4. Epub 2004 Sep 29.
2
Identification of the gene encoding the tryptophan synthase beta-subunit from Chlamydomonas reinhardtii.莱茵衣藻色氨酸合成酶β亚基编码基因的鉴定。
Plant Physiol. 1998 Jun;117(2):455-64. doi: 10.1104/pp.117.2.455.
3
Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.嗜热栖热菌（Thermus thermophilus）的嘧啶生物合成基因（pyrE和pyrF）
Appl Environ Microbiol. 1996 Jun;62(6):2191-4. doi: 10.1128/aem.62.6.2191-2194.1996.
4
Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cells.嗜热栖热菌（Thermus thermophilus）基因在大肠杆菌细胞中的过表达。
J Bacteriol. 1994 May;176(9):2767-70. doi: 10.1128/jb.176.9.2767-2770.1994.
5
Synechocystis PCC 6803 contains a single gene for the beta subunit of tryptophan synthase with strong homology to the trpB genes of Arabidopsis and maize (Zea mays L.).集胞藻PCC 6803含有一个编码色氨酸合酶β亚基的基因，该基因与拟南芥和玉米（Zea mays L.）的trpB基因具有高度同源性。
Plant Physiol. 1994 Feb;104(2):461-6. doi: 10.1104/pp.104.2.461.
6
Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.嗜热栖热放线菌中pyrE基因的分子克隆。
J Bacteriol. 1995 Aug;177(15):4540-3. doi: 10.1128/jb.177.15.4540-4543.1995.
7
Tryptophan gene cluster of Methanobacterium thermoautotrophicum Marburg: molecular cloning and nucleotide sequence of a putative trpEGCFBAD operon.嗜热自养甲烷杆菌马尔堡菌株的色氨酸基因簇：假定的trpEGCFBAD操纵子的分子克隆及核苷酸序列
J Bacteriol. 1991 Aug;173(16):5017-23. doi: 10.1128/jb.173.16.5017-5023.1991.
8
Molecular cloning of a ribonuclease H (RNase HI) gene from an extreme thermophile Thermus thermophilus HB8: a thermostable RNase H can functionally replace the Escherichia coli enzyme in vivo.嗜热栖热菌HB8核糖核酸酶H（RNase HI）基因的分子克隆：一种耐热核糖核酸酶H在体内可功能性替代大肠杆菌的该种酶。
Nucleic Acids Res. 1991 Aug 25;19(16):4443-9. doi: 10.1093/nar/19.16.4443.
9
Molecular cloning of the isocitrate dehydrogenase gene of an extreme thermophile, Thermus thermophilus HB8.嗜热栖热菌HB8异柠檬酸脱氢酶基因的分子克隆
Appl Environ Microbiol. 1992 Jan;58(1):93-8. doi: 10.1128/aem.58.1.93-98.1992.
10
Structure of the phenylalanyl-tRNA synthetase genes from Thermus thermophilus HB8 and their expression in Escherichia coli.嗜热栖热菌HB8苯丙氨酰-tRNA合成酶基因的结构及其在大肠杆菌中的表达。
Nucleic Acids Res. 1992 Aug 25;20(16):4173-8. doi: 10.1093/nar/20.16.4173.
本文引用的文献
1
Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium.大肠杆菌和鼠伤寒沙门氏菌中色氨酸合成酶B基因的核苷酸序列。
J Mol Biol. 1980 Oct 5;142(4):489-502. doi: 10.1016/0022-2836(80)90259-4.
2
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.通过在大肠杆菌中进行DNA融合和克隆分析基因控制信号。
J Mol Biol. 1980 Apr;138(2):179-207. doi: 10.1016/0022-2836(80)90283-1.
3
A DNA methylase from Thermus thermophilus HB8.来自嗜热栖热菌HB8的一种DNA甲基化酶。
J Biochem. 1980 Sep;88(3):737-47. doi: 10.1093/oxfordjournals.jbchem.a133026.
4
5
Translational coupling of the trpB and trpA genes in the Escherichia coli tryptophan operon.大肠杆菌色氨酸操纵子中trpB和trpA基因的翻译偶联
J Bacteriol. 1984 Feb;157(2):363-7. doi: 10.1128/jb.157.2.363-367.1984.
6
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
7
Direct plasmid transfer from replica-plated E. coli colonies to competent B. subtilis cells. Identification of an E. coli clone carrying the hisH and tyrA genes of B. subtilis.从影印接种的大肠杆菌菌落直接将质粒转移至感受态枯草芽孢杆菌细胞。鉴定携带枯草芽孢杆菌hisH和tyrA基因的大肠杆菌克隆。
Mol Gen Genet. 1984;195(1-2):57-61. doi: 10.1007/BF00332724.
8
Proteins from thermophilic microorganisms.来自嗜热微生物的蛋白质。
Bacteriol Rev. 1973 Sep;37(3):320-42. doi: 10.1128/br.37.3.320-342.1973.
9
Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.嗜热栖热菌及其他栖热菌属的极端嗜热菌的遗传转化
J Bacteriol. 1986 Apr;166(1):338-40. doi: 10.1128/jb.166.1.338-340.1986.
10
Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity.嗜热栖热菌苹果酸脱氢酶基因的核苷酸序列及其指导酶活性增加的突变
J Biol Chem. 1986 Oct 25;261(30):14178-83.
Zotero 插件