一种用于嗜热栖热菌（Thermus thermophilus）这种极端嗜热菌的质粒载体。
A plasmid vector for an extreme thermophile, Thermus thermophilus.
作者信息
Koyama Y, Arikawa Y, Furukawa K
机构信息
Fermentation Research Institute, AIST, MITI, Tsukuba Science City, Ibaraki, Japan.
出版信息
FEMS Microbiol Lett. 1990 Oct;60(1-2):97-101. doi: 10.1016/0378-1097(90)90352-q.
The host-vector system for an extreme thermophile, Thermus thermophilus HB27, was developed. The host strain has a mutation in tryptophan synthetase gene (trpB), and the mutation was determined to be a missense mutation by DNA sequence analysis. A Thermus-E. coli shuttle vector pYK109 was constructed. pYK109 consists of Thermus cryptic plasmid pTT8, tryptophan synthetase gene (trpB) of Thermus T2 and E. coli plasmid vector pUC13. pYK109 transformed T. thermophilus HB27 trpB5 to Trp+ at a frequency of 10(6) transformants per microgram DNA.
开发了一种适用于嗜热栖热菌HB27这种极端嗜热菌的宿主-载体系统。宿主菌株在色氨酸合成酶基因（trpB）中存在突变，通过DNA序列分析确定该突变是错义突变。构建了一种嗜热栖热菌-大肠杆菌穿梭载体pYK109。pYK109由嗜热栖热菌隐蔽质粒pTT8、嗜热栖热菌T2的色氨酸合成酶基因（trpB）和大肠杆菌质粒载体pUC13组成。pYK109以每微克DNA产生10⁶个转化子的频率将嗜热栖热菌HB27 trpB5转化为色氨酸合成酶阳性（Trp⁺）。
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