Harichane Y, Hirata A, Dimitrova-Nakov S, Granja I, Goldberg A, Kellermann O, Poliard A
INSERM UMR-S, UFR Biomédicales des Saints-Pères, Université Paris Descartes, France.
Adv Dent Res. 2011 Jul;23(3):307-12. doi: 10.1177/0022034511405322.
Mesenchymal stem cells are present in the dental pulp. They have been shown to contribute to dentin-like tissue formation in vitro and to participate in bone repair after a mandibular lesion. However, their capacity to contribute efficiently to reparative dentin formation after pulp lesion has never been explored. After pulp exposure, we have identified proliferative cells within 3 zones. In the crown, zone I is near the cavity, and zone II corresponds to the isthmus between the mesial and central pulp. In the root, zone III, near the apex, at a distance from the inflammatory site, contains mitotic stromal cells which may represent a source of progenitor cells. Stem-cell-based strategies are promising treatments for tissue injury in dentistry. Our experiments focused on (1) location of stem cells induced to leave their quiescent state early after pulp injury and (2) implantation of pulp progenitors, a substitute for classic endodontic treatments, paving the way for pulp stem-cell-based therapies.
间充质干细胞存在于牙髓中。研究表明，它们能在体外促进牙本质样组织形成，并参与下颌骨损伤后的骨修复。然而，它们在牙髓损伤后有效促进修复性牙本质形成的能力从未被探究过。牙髓暴露后，我们在三个区域内鉴定出了增殖细胞。在牙冠部，I区靠近髓腔，II区对应于近中牙髓和中央牙髓之间的峡部。在根部，III区靠近根尖，远离炎症部位，含有有丝分裂的基质细胞，这些细胞可能是祖细胞的来源。基于干细胞的策略是治疗牙科组织损伤的有前景的方法。我们的实验聚焦于：（1）牙髓损伤后早期被诱导离开静止状态的干细胞的位置；（2）牙髓祖细胞的植入，这是一种替代传统牙髓治疗的方法，为基于牙髓干细胞的治疗铺平了道路。
