Goldberg M
UMR-S, INSERM, Université Paris Descartes, UFR, Biomédicale des Saints Pères, France.
Adv Dent Res. 2011 Jul;23(3):270-4. doi: 10.1177/0022034511405385.
Differences between pulp repair and regeneration guide different strategic options. After mild carious dentin lesions, odontoblasts and Hoehl's cells are implicated in the formation of reactionary dentin. Reparative dentin formation and/or pulp regeneration after partial degradation is under the control of pulp progenitors. A series of questions arise from recent researches on tissue engineering. In this series of questions, we compare the therapeutic potential of pluripotent embryonic and adult stem cells, both being used in cell-based dental therapies. Crucial questions arise on the origin of stem cells and the localization of niches of progenitors in adult teeth. Circulating progenitor cells may also be candidate for promoting pulp regeneration. Then, we focus on strategies allowing efficient progenitors recruitment. Along this line, we compare the potential of embryonic stem cells versus adult stem cells. Re-programming adult pulp cells to become induced pluripotent stem cells constitute another option. Genes, transcription factors and growth factors may be used to stimulate the differentiation cascade. Extracellular matrix molecules or some bioactive specific domains after enzymatic cleavage may also contribute to the formation of an artificial pulp and ultimately to its mineralization.
牙髓修复与再生之间的差异引导了不同的策略选择。在轻度龋损牙本质病变后，成牙本质细胞和赫氏细胞参与反应性牙本质的形成。部分降解后的修复性牙本质形成和/或牙髓再生受牙髓祖细胞控制。近期组织工程研究引发了一系列问题。在这一系列问题中，我们比较了多能胚胎干细胞和成人干细胞在基于细胞的牙科治疗中的治疗潜力。关于干细胞的起源以及成体牙齿中祖细胞龛的定位出现了关键问题。循环祖细胞也可能是促进牙髓再生的候选细胞。然后，我们关注能够有效招募祖细胞的策略。沿着这条线，我们比较胚胎干细胞与成人干细胞的潜力。将成人牙髓细胞重编程为诱导多能干细胞是另一种选择。基因、转录因子和生长因子可用于刺激分化级联反应。酶解后的细胞外基质分子或一些生物活性特定结构域也可能有助于人工牙髓的形成并最终促进其矿化。
