Singh Vikas Kumar, Upadhyay Priti, Sinha Pallavi, Mall Ashish Kumar, Jaiswal Sanjay Kumar, Singh Atul, Ellur Ranjith Kumar, Biradar Sunil, Sundaram R M, Singh Sukhpal, Ahmed Ilyas, Mishra B, Singh A K, Kole C
Allahabad Agricultural Institute-Deemed University, Allahabad 211 007, India.
J Genet. 2011 Apr;90(1):11-9. doi: 10.1007/s12041-011-0018-5.
A set of morphological traits and SSR markers were used to determine the genetic relationship among 12 elite thermosensitive genic male sterile (TGMS) lines developed at three different research institutions of India. Agro-morphological data recorded on 20 morphological traits revealed a wide base of genetic variation and a set of four morphological traits could distinguish most of the TGMS lines. Analysis with 30 SSR markers (20 EST-SSRs and 10 genomic SSRs) revealed 27 markers to be polymorphic, amplifying a total of 83 alleles. Each SSR marker amplified 2-6 alleles with an average of 2.76 alleles per marker and a PIC value varying from 0.54 to 0.96. Cluster analysis based on SSR and morphological data clearly differentiated the lines according to their source of origin. Correlation analysis between morphological and molecular data revealed a very poor association (r = 0.06), which could be attributed to selection pressure, genetic drift, sampling error and unknown relationship among related lines. The SSR markers discriminated the genotypes distinctly and quantified the genetic diversity precisely among the TGMS lines. Data on the yield per plant indicated that the genotypes grouping under a similar cluster showed same heterotic behaviour as compared to the genotypes from different clusters when crossed to similar pollinators.
利用一组形态性状和SSR标记来确定印度三个不同研究机构培育的12个优良温敏雄性不育（TGMS）系之间的遗传关系。对20个形态性状记录的农艺形态数据显示出广泛的遗传变异基础，一组四个形态性状可以区分大多数TGMS系。用30个SSR标记（20个EST-SSR和10个基因组SSR）进行分析，发现27个标记具有多态性，共扩增出83个等位基因。每个SSR标记扩增出2-6个等位基因，平均每个标记2.76个等位基因，PIC值在0.54至0.96之间。基于SSR和形态数据的聚类分析根据系的起源明确区分了这些系。形态和分子数据之间的相关性分析显示关联非常弱（r = 0.06），这可能归因于选择压力、遗传漂变、抽样误差以及相关系之间未知的关系。SSR标记能清晰地区分基因型，并精确量化TGMS系之间的遗传多样性。单株产量数据表明，当与相似的授粉者杂交时，同一聚类下的基因型与来自不同聚类的基因型相比表现出相同的杂种优势行为。
