mRNA 展示选择相对于其他选择技术在研究蛋白质-蛋白质相互作用方面的优势。
Advantages of mRNA display selections over other selection techniques for investigation of protein-protein interactions.
机构信息
University of North Carolina, Chapel Hill, NC 27599-7568, USA.
出版信息
Expert Rev Proteomics. 2011 Jun;8(3):335-46. doi: 10.1586/epr.11.15.
Abstract
mRNA display is a genotype-phenotype conjugation method that allows for amplification-based, iterative rounds of in vitro selection to be applied to peptides and proteins. mRNA display can be used to display both long natural protein and short synthetic peptide libraries with unusually high diversities for the investigation of protein-protein interactions. Here, we summarize the advantages of mRNA display by comparing it with other widely used peptide or protein-selection techniques, and discuss various applications of this technique in studying protein-protein interactions.
摘要
mRNA 展示是一种基因型-表型结合方法，允许对肽和蛋白质进行基于扩增的、迭代的体外选择。mRNA 展示可用于展示具有异常高多样性的长天然蛋白质和短合成肽文库，用于研究蛋白质-蛋白质相互作用。在这里，我们通过与其他广泛使用的肽或蛋白质选择技术进行比较，总结了 mRNA 展示的优点，并讨论了该技术在研究蛋白质-蛋白质相互作用中的各种应用。
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