Nakajima K, Shibayama T, Kato S, Kurimura T, Hirai K
Department of Pathology, Osaka University, Japan.
J Gen Virol. 1990 Aug;71 ( Pt 8):1807-10. doi: 10.1099/0022-1317-71-8-1807.
The post-translational events leading from the precursor to the processed forms of a glycoprotein with an Mr of 28K to 32K (gp27/32) of Marek's disease virus (MDV) serotype 2 were examined with pulse-chase experiments and treatment with tunicamycin and monensin. Cell-free translation of infected cell mRNA followed by immunoprecipitation analysis suggested that a polypeptide with a size of 22K is the initial precursor. Experiments with endo-beta-N-acetylglucosaminidase H and endo-beta-N-acetylglucosaminidase F indicated that gp28/32 contains mostly N-linked oligosaccharides of the complex type. These studies showed that 22K, the initial product, is then processed through intermediates to the 28K to 32K form.
利用脉冲追踪实验以及衣霉素和莫能菌素处理，研究了2型马立克氏病病毒（MDV）中糖蛋白前体转变为分子量为28K至32K（gp27/32）的加工形式的翻译后事件。对感染细胞的mRNA进行无细胞翻译，然后进行免疫沉淀分析，结果表明，大小为22K的多肽是初始前体。用内切β-N-乙酰氨基葡萄糖苷酶H和内切β-N-乙酰氨基葡萄糖苷酶F进行的实验表明，gp28/32主要含有复合型N-连接寡糖。这些研究表明，初始产物22K随后通过中间体加工成28K至32K的形式。
