Inumaru S, Roy P
Virology. 1987 Apr;157(2):472-9. doi: 10.1016/0042-6822(87)90289-3.
DNA representing RNA segment 2 of bluetongue virus (BTV) serotype 10, corresponding to the gene that codes for the BTV neutralization antigen VP2, has been inserted into a baculovirus transfer vector in lieu of the 5' coding region of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus (AcNPV). After cotransfection of Spodoptera frugiperda cells with wild-type AcNPV DNA in the presence of the derived recombinant transfer vector DNA, polyhedrin-negative recombinant baculoviruses were recovered. When S. frugiperda cells were infected with one of these recombinant viruses, a protein that was similar in size and antigenic properties to the BTV VP2 protein was synthesized. Antibodies raised in mice or rabbits to the baculovirus expressed VP2 protein neutralized the infectivity of BTV-10 virus and to lesser extents BTV serotype 11 and 17 viruses but not BTV-13 virus.
代表蓝舌病病毒10型（BTV-10）RNA片段2的DNA，对应于编码BTV中和抗原VP2的基因，已被插入杆状病毒转移载体，取代了苜蓿银纹夜蛾核型多角体病毒（AcNPV）多角体蛋白基因的5'编码区。在衍生的重组转移载体DNA存在下，将草地贪夜蛾细胞与野生型AcNPV DNA共转染后，获得了多角体蛋白阴性的重组杆状病毒。当用这些重组病毒之一感染草地贪夜蛾细胞时，合成了一种大小和抗原特性与BTV VP2蛋白相似的蛋白质。用杆状病毒表达的VP2蛋白在小鼠或兔中产生的抗体中和了BTV-10病毒的感染性，并在较小程度上中和了BTV 11型和17型病毒，但不能中和BTV-13病毒。
