Inumaru S, Ghiasi H, Roy P
J Gen Virol. 1987 Jun;68 ( Pt 6):1627-35. doi: 10.1099/0022-1317-68-6-1627.
DNA representing RNA segment 3 of bluetongue virus (BTV) serotype 17, corresponding to the gene that codes for a group-specific antigen VP3, has been inserted into a baculovirus transfer vector in lieu of the 5' coding region of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus (AcNPV). After cotransfection of Spodoptera frugiperda cells with wild-type AcNPV DNA in the presence of the derived recombinant transfer vector DNA, polyhedrin-negative recombinant baculoviruses were recovered. When S. frugiperda cells were infected with one of these recombinant viruses, a protein that was similar in size and antigenic properties to the BTV VP3 protein was synthesized. Antibodies raised in mice or rabbits to the baculovirus-expressed VP3 protein immunoprecipitated the VP3 protein of BTV-17 as well as that of BTV-10. The expressed antigen reacted with antisera representing four U.S.A. BTV serotypes in an indirect ELISA test.
代表蓝舌病毒（BTV）17型RNA片段3的DNA，对应于编码组特异性抗原VP3的基因，已被插入杆状病毒转移载体中，取代了苜蓿银纹夜蛾核型多角体病毒（AcNPV）多角体蛋白基因的5'编码区。在衍生的重组转移载体DNA存在下，将野生型AcNPV DNA与草地贪夜蛾细胞共转染后，回收了多角体蛋白阴性的重组杆状病毒。当用这些重组病毒之一感染草地贪夜蛾细胞时，合成了一种大小和抗原特性与BTV VP3蛋白相似的蛋白质。用杆状病毒表达的VP3蛋白在小鼠或兔中产生的抗体免疫沉淀了BTV-17以及BTV-10的VP3蛋白。在间接ELISA试验中，表达的抗原与代表四种美国BTV血清型的抗血清发生反应。
