利用铕发光的时间门控测量分析小体积精液样本中的柠檬酸盐。

Analysis of citrate in low-volume seminal fluid samples using a time-gated measurement of europium luminescence.

机构信息

Department of Chemistry, Durham University, South Road, Durham DH13LE, UK.

出版信息

J Pharm Biomed Anal. 2011 Sep 10;56(2):352-8. doi: 10.1016/j.jpba.2011.05.023. Epub 2011 May 20.

DOI:10.1016/j.jpba.2011.05.023

Abstract

This work aims to develop and validate a rapid analytical method that enables the measurement of citrate in seminal fluid samples. Samples were obtained from men who were within 9 weeks of a vasectomy operation. Two age ranges were examined, between 40-43 and 50-53 years old, with nearly 100 samples in each case. No patient clinical history was available for this anonymous study, simulating a random screening cohort. The concentration of citrate in 0.5 μL seminal fluid samples was assessed, using a europium emission luminescence method. This involves the ratiometric analysis of two well-separated europium(III) emission bands. Spectral data were obtained using a time-gated spectrometer whose construction and modification is described. Citrate values were confirmed by independent measurements using a citrate lyase enzymatic assay and by 700 MHz ¹H NMR analysis of the seminal fluid. Citrate concentrations were not statistically different between age groups and averaged 35.0(±14.6) mM for the 40-43 group, and 28.2(±12.7) mM for the 50-53 cohort; in each case a polymodal distribution was observed.

摘要

本工作旨在开发和验证一种快速分析方法，以测量精液样本中的柠檬酸。样本取自接受输精管切除术不到 9 周的男性。检查了两个年龄范围，分别为 40-43 岁和 50-53 岁，每个年龄范围都有近 100 个样本。由于这是一项匿名研究，没有患者的临床病史，模拟了一个随机筛查队列。使用铕发射发光法评估 0.5μL 精液样本中柠檬酸的浓度。这涉及两个分离良好的铕(III)发射带的比率分析。使用时间门控光谱仪获得光谱数据，其构造和修改有所描述。通过使用柠檬酸裂解酶酶测定法和对精液的 700 MHz ¹H NMR 分析对柠檬酸值进行了独立测量和确认。在年龄组之间，柠檬酸浓度没有统计学差异，40-43 岁组平均为 35.0(±14.6)mM，50-53 岁组平均为 28.2(±12.7)mM；在每种情况下，都观察到多峰分布。