MOE Key Laboratory of Protein Sciences, Department of Biological Sciences and Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Bioresour Technol. 2011 Sep;102(17):8130-6. doi: 10.1016/j.biortech.2011.05.068. Epub 2011 May 30.
An unsterile and continuous fermentation process was developed based on a halophilic bacterium termed Halomonas TD01 isolated from a salt lake in Xinjiang, China. The strain reached 80 g/L cell dry weight containing 80% poly(3-hydroxybutyrate) (PHB) on glucose salt medium during a 56 h fed-batch process. In a 14-day open unsterile and continuous process, the cells grew to an average of 40 g/L cell dry weight containing 60% PHB in the first fermentor with glucose salt medium. Continuous pumping of cultures from the first fermentor to the second fermentor containing the nitrogen-deficient glucose salt medium diluted the cells but allowed them to maintain a PHB level of between 65% and 70% of cell dry weight. Glucose to PHB conversions were between 20% and 30% in the first fermentor and above 50% in the second one. This unsterile and continuous fermentation process opens a new area for reducing the cost in polyhydroxyalkanoates production.
一种非无菌连续发酵工艺是基于从中国新疆盐湖中分离出的嗜盐菌 Halomonas TD01 开发的。在 56 小时的分批补料过程中，该菌株在葡萄糖盐培养基中达到 80 g/L 的细胞干重，其中含有 80%的聚（3-羟基丁酸酯）（PHB）。在 14 天的开放式非无菌连续过程中，细胞在第一个发酵罐中以葡萄糖盐培养基生长至平均 40 g/L 的细胞干重，其中含有 60%的 PHB。将培养物从第一个发酵罐连续泵送到含有氮缺乏的葡萄糖盐培养基的第二个发酵罐中，虽然会稀释细胞，但可以使它们保持 65%至 70%的 PHB 细胞干重水平。在第一个发酵罐中，葡萄糖到 PHB 的转化率在 20%至 30%之间，而在第二个发酵罐中则超过 50%。这种非无菌和连续发酵工艺为降低聚羟基烷酸酯生产成本开辟了新的领域。
