Lee E B, Hayman M J
Department of Microbiology, State University of New York, Stony Brook 11794.
Oncogene. 1990 Aug;5(8):1165-72.
The role of the extracellular domain of the v-erbB oncogene in the transformation of chicken fibroblasts and erythroblasts has been examined. A virus was isolated in which the complete extracellular domain and the transmembrane domain of the v-erbB were replaced with sequences from the gag and env genes. The resulting virus, GEE35, was capable of transforming both fibroblasts and erythroblasts as efficiently as wild-type v-erbB. Biochemical and immunochemical analysis of the v-erbB protein encoded by GEE35 revealed two proteins of apparent molecular weights of approximately 130,000 daltons. One of these proteins was an N-linked glycosylated membrane protein, whereas the other was cytoplasmic. The possible roles of either of these two proteins in transformation is discussed.
已对v-erbB癌基因的细胞外结构域在鸡成纤维细胞和红细胞转化中的作用进行了研究。分离出一种病毒，其中v-erbB的完整细胞外结构域和跨膜结构域被来自gag和env基因的序列所取代。产生的病毒GEE35能够像野生型v-erbB一样高效地转化成纤维细胞和红细胞。对GEE35编码的v-erbB蛋白进行的生化和免疫化学分析揭示了两种表观分子量约为130,000道尔顿的蛋白质。其中一种蛋白质是N-连接糖基化膜蛋白，而另一种是细胞质蛋白。讨论了这两种蛋白质中任何一种在转化中的可能作用。
