Scotting P, Vennstrom B, Jansen M, Graf T, Beug H, Hayman M J
Imperial Cancer Research Fund Laboratories, St Bartholomews Hospital, London.
Oncogene Res. 1987 Aug;1(3):265-78.
The genome of the avian erythroblastosis virus temperature sensitive mutant ts34 was cloned from a cell line that was shown to contain a single integrated copy of the virus. The mutation was localized to the v-erbB gene by making chimeric viruses between the mutant genome and that of wildtype. Sequencing of the mutant v-erbB gene revealed a single amino acid change of a histidine to an aspartate residue at a position equivalent to amino-acid 826 of the human epidermal growth factor receptor. Interestingly this is the identical mutation to that recently reported for another temperature sensitive mutant ts167, indicating that this may be a hot spot for mutations in the v-erbB gene that give rise to ts transformation mutants. In addition, the different biological phenotypes of ts34 and ts167 are suggested to be due to an additional mutation in v-erbA in ts167.
禽成红细胞增多症病毒温度敏感突变体ts34的基因组是从一个细胞系中克隆出来的，该细胞系被证明含有该病毒的单个整合拷贝。通过在突变体基因组和野生型基因组之间构建嵌合病毒，将突变定位到v-erbB基因。对突变的v-erbB基因进行测序发现，在相当于人类表皮生长因子受体氨基酸826的位置，有一个组氨酸残基变为天冬氨酸残基的单氨基酸变化。有趣的是，这与最近报道的另一个温度敏感突变体ts167的突变相同，表明这可能是v-erbB基因中导致ts转化突变体的突变热点。此外，ts34和ts167不同的生物学表型被认为是由于ts167的v-erbA中存在额外的突变。
