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高通量测序获得了来自秘鲁东北部的特有物种(兰科)的完整质体基因组。

High-throughput sequencing yields the complete plastid genome of the endemic species (Orchidaceae) from northeastern Peru.

作者信息

Carrion Jois V, Perez Jhordy, Tineo Daniel, Calderon Martha S, Garcia Ligia, Oliva Manuel, Gamarra Torres Oscar, Bustamante Danilo E

机构信息

Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza, Chachapoyas, Peru.

Instituto de Investigación en Ingeniería Ambiental (INAM), Facultad de Ingeniería Civil y Ambiental (FICIAM), Universidad Nacional Toribio Rodríguez de Mendoza, Chachapoyas, Peru.

出版信息

Mitochondrial DNA B Resour. 2024 Sep 2;9(9):1175-1180. doi: 10.1080/23802359.2024.2397979. eCollection 2024.

DOI:10.1080/23802359.2024.2397979
PMID:39247500
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11378673/
Abstract

is a species of orchid endemic to the Amazonas and San Martín regions. Unfortunately, its excessive extraction has made it a critically endangered species. In this study, we performed next-generation sequencing of (GenBank accession number OR348669) and assembled its complete chloroplast genome. The complete chloroplast genome of is A + T-rich (64.3%), measuring 152,918 bp in length. This plastid genome contains a total of 124 genes (77 protein-coding genes, 39 tRNAs, and eight rRNAs) and five pseudogenes, including a pair of inverted repeats (IRs) 25,116 bp in size and separated by a large single-copy (LSC) region of 89,216 bp and a small single-copy (SSC) region of 13,470 bp. This genome has a typical quadripartite organization following the structure of other Orchidaceae plastomes. Phylogenetic analyses revealed the close relationship between and This study contributes to the understanding of the phylogenetic relationships of the monophyletic group Cypripedioideae.

摘要

是一种兰花,为亚马逊地区和圣马丁地区所特有。不幸的是,其过度采挖使其成为极度濒危物种。在本研究中,我们对(GenBank登录号OR348669)进行了二代测序,并组装了其完整的叶绿体基因组。的完整叶绿体基因组富含A+T(64.3%),长度为152,918 bp。该质体基因组共包含124个基因(77个蛋白质编码基因、39个tRNA和8个rRNA)以及5个假基因,包括一对大小为25,116 bp的反向重复序列(IR),它们被一个89,216 bp的大单拷贝(LSC)区域和一个13,470 bp的小单拷贝(SSC)区域隔开。该基因组具有典型的四分体结构,与其他兰科植物质体基因组结构一致。系统发育分析揭示了与之间的密切关系。本研究有助于理解单系类群杓兰亚科的系统发育关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abba/11378673/ac3b7e4d3f26/TMDN_A_2397979_F0003_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abba/11378673/94d95b040f35/TMDN_A_2397979_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abba/11378673/5e4b0944b4ae/TMDN_A_2397979_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abba/11378673/ac3b7e4d3f26/TMDN_A_2397979_F0003_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abba/11378673/94d95b040f35/TMDN_A_2397979_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abba/11378673/5e4b0944b4ae/TMDN_A_2397979_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abba/11378673/ac3b7e4d3f26/TMDN_A_2397979_F0003_C.jpg

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Comparative Analysis of Complete Chloroplast Genomes of Nine Species of (Lauraceae): Hypervariable Regions, Positive Selection, and Phylogenetic Relationships.
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Genes (Basel). 2022 Aug 28;13(9):1550. doi: 10.3390/genes13091550.
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Complete Chloroplast Genome of and Phylogenetic Relationships among Orchidaceae.兰科植物的叶绿体全基因组及系统发育关系
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