Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011 Japan.
Plant Cell Physiol. 2011 Aug;52(8):1330-9. doi: 10.1093/pcp/pcr077. Epub 2011 Jun 16.
Flowering plants produce multicellular gametophytes through an elaborate regulation of gametogenesis. During female and male gametogenesis in Arabidopsis thaliana, sporogenous cells differentiate and undergo meiosis to produce megaspores and microspores, which in turn go through mitosis to develop into multicellular gametophytes. Here we report that the Arabidopsis spliceosomal protein, SPLICEOSOME-ASSOCIATED PROTEIN 130 (AtSAP130), is required for proper reproduction. AtSAP130 is encoded by two genes, AtSAP130a and AtSAP130b. Plants with reduced expression of the AtSAP130 genes, induced by RNA interference, showed a defect in fertilization. Besides functional impairment observed in the female reproductive organs, analysis focusing on pollen development revealed defects in the transition from the microspore to the bicellular stage. Our results suggest that AtSAP130a and AtSAP130b play an indispensable role in specific spatiotemporal events in reproduction.
开花植物通过配子发生的精细调控产生多细胞配子体。在拟南芥的雌性和雄性配子发生过程中，孢子体细胞分化并经历减数分裂产生大孢子和小孢子，小孢子继而经过有丝分裂发育成多细胞配子体。在这里，我们报告称，拟南芥剪接体蛋白 SPLICEOSOME-ASSOCIATED PROTEIN 130（AtSAP130）是正常繁殖所必需的。AtSAP130 由两个基因 AtSAP130a 和 AtSAP130b 编码。通过 RNA 干扰诱导的 AtSAP130 基因表达降低的植物在受精过程中表现出缺陷。除了在雌性生殖器官中观察到的功能障碍外，对花粉发育的分析表明，从小孢子到双核阶段的转变存在缺陷。我们的结果表明，AtSAP130a 和 AtSAP130b 在生殖过程中的特定时空事件中发挥不可或缺的作用。
