Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan.
Plant Cell. 2013 Jun;25(6):2056-69. doi: 10.1105/tpc.113.111922. Epub 2013 Jun 14.
Pre-mRNA splicing is a critical process in gene expression in eukaryotic cells. A multitude of proteins are known to be involved in pre-mRNA splicing in plants; however, the physiological roles of only some of these have been examined. Here, we investigated the developmental roles of a pre-mRNA splicing factor by analyzing root initiation defective1-1 (rid1-1), an Arabidopsis thaliana mutant previously shown to have severe defects in hypocotyl dedifferentiation and de novo meristem formation in tissue culture under high-temperature conditions. Phenotypic analysis in planta indicated that RID1 is differentially required during development and has roles in processes such as meristem maintenance, leaf morphogenesis, and root morphogenesis. RID1 was identified as encoding a DEAH-box RNA helicase implicated in pre-mRNA splicing. Transient expression analysis using intron-containing reporter genes showed that pre-mRNA splicing efficiency was affected by the rid1 mutation, which supported the presumed function of RID1 in pre-mRNA splicing. Our results collectively suggest that robust levels of pre-mRNA splicing are critical for several specific aspects of plant development.
前体 mRNA 剪接是真核细胞中基因表达的一个关键过程。已知有多种蛋白质参与植物前体 mRNA 的剪接；然而，这些蛋白质的生理作用只有一部分得到了研究。在这里，我们通过分析根起始缺陷 1-1（rid1-1）突变体，研究了前体 mRNA 剪接因子的发育作用。rid1-1 是拟南芥突变体，先前的研究表明，在高温条件下的组织培养中，该突变体在胚轴去分化和从头分生组织形成方面存在严重缺陷。在植物体内的表型分析表明，RID1 在发育过程中具有不同的需求，并在分生组织维持、叶片形态发生和根形态发生等过程中发挥作用。RID1 被鉴定为编码一种 DEAH 盒 RNA 解旋酶，该酶参与前体 mRNA 的剪接。使用内含子报告基因的瞬时表达分析表明，前体 mRNA 剪接效率受到 rid1 突变的影响，这支持了 RID1 在 pre-mRNA 剪接中的假定功能。我们的研究结果表明，前体 mRNA 剪接的稳健水平对植物发育的几个特定方面至关重要。
