PET Centre, Aarhus University Hospital, Aarhus, Denmark.
J Nucl Med. 2011 Jul;52(7):1119-24. doi: 10.2967/jnumed.111.088278. Epub 2011 Jun 16.
There is an unmet clinical need for an imaging method for quantification of hepatic blood perfusion. The purpose of the present study was to develop and validate a PET method using blood-to-cell clearance (K(1)) of (18)F-FDG, 3-O-(11)C-methylglucose ((11)C-MG), or 2-(18)F-fluoro-2-deoxy-D-galactose ((18)F-FDGal) as a measure of hepatic blood perfusion without the need for portal venous blood samples. We aimed to make the method as simple as possible with the prospect of future application to clinical studies. For this purpose, we examined the possibility of using a 3-min data acquisition and a model-derived dual input calculated from measurements of radioactivity concentrations in a peripheral artery.
Pigs (40 kg) underwent dynamic PET of the liver with (18)F-FDG, (11)C-MG, or (18)F-FDGal with simultaneous measurements of time-activity curves in blood sampled from a femoral artery and the portal vein (PV); blood flow rates were measured in the hepatic artery (HA) and PV by transit-time flow meters. Two input functions were compared: A measured dual input and a model-derived dual input, the latter with the PV time-activity curve estimated from the measured arterial time-activity curve and a previously validated 1-parametric PV model. (K(1)) was estimated for each tracer by fitting compartmental models to the data, comparing 3-min and 60-min data acquisitions and the 2 dual-input time-activity curves.
Agreement between (K(1)) estimated using the measured and the model-derived dual input was good for all 3 tracers. For (18)F-FDG and (11)C-MG, (K(1)) (3-min data acquisition, model-derived dual input, and 1-tissue compartmental model) correlated to the measured blood perfusion (P = 0.01 and P = 0.07, respectively). For (18)F-FDGal, the correlation was not significant.
A simplified method for quantification of hepatic blood perfusion using 3-min dynamic (18)F-FDG PET or (11)C-MG PET with blood sampling from only a peripheral artery was developed. Parametric (K(1)) images were constructed and showed homogeneous blood perfusion in these normal livers.
开发并验证一种使用（18）F-FDG、3-O-（11）C-甲基葡萄糖（（11）C-MG）或 2-（18）F-氟-2-脱氧-D-半乳糖（（18）F-FDGal）的血到细胞清除率（K（1））作为肝血灌注的测量方法，而无需门静脉血样。我们旨在使该方法尽可能简单，以便将来应用于临床研究。为此，我们研究了使用 3 分钟数据采集和从外周动脉放射性浓度测量中计算出的模型衍生的双输入的可能性。
猪（40 公斤）进行肝动态 PET，用（18）F-FDG、（11）C-MG 或（18）F-FDGal 进行，同时从股动脉和门静脉（PV）采集血液的时间-活性曲线进行测量；肝动脉（HA）和 PV 的血流率通过瞬变流量计测量。比较了两种输入函数：一种是测量得到的双输入，另一种是模型衍生的双输入，后者是通过将测量得到的动脉时间-活性曲线与以前验证的 1 参数 PV 模型估计的 PV 时间-活性曲线结合来计算的。通过将房室模型拟合到数据中，比较 3 分钟和 60 分钟的数据采集和 2 种双输入时间-活性曲线，为每个示踪剂估计 K（1）。
对于所有 3 种示踪剂，使用测量得到的和模型衍生的双输入估计的 K（1）之间的一致性很好。对于（18）F-FDG 和（11）C-MG，（K（1））（3 分钟数据采集、模型衍生的双输入和 1 组织房室模型）与测量的血流灌注相关（分别为 P = 0.01 和 P = 0.07）。对于（18）F-FDGal，相关性不显著。
开发了一种使用仅从外周动脉采集血液的 3 分钟动态（18）F-FDG PET 或（11）C-MG PET 定量肝血灌注的简化方法。构建了参数 K（1）图像，并显示这些正常肝脏的血液灌注均匀。
