USDA-ARS, Plant Genetic Resources Conservation Unit, Griffin, GA 30223, USA.
Biochem Genet. 2011 Dec;49(11-12):748-59. doi: 10.1007/s10528-011-9447-3. Epub 2011 Jun 17.
A panel of 55 peanut lines was analyzed for fatty acid composition with gas chromatography and also genotyped with SNP markers from the FAD2 genes by real-time PCR. Significant variation in fatty acid composition was identified, and the ratio of oleic acid to linoleic acid (O/L) ranged from 1.23 to 56.45. In terms of the FAD2 gene mutation, the assayed lines were classified into four genotypes: wild type (Ol(1)Ol(1)Ol(2)Ol(2)), single functional homozygous mutation on the A genome (ol(1)ol(1)Ol(2)Ol(2)), single functional homozygous mutation on the B genome (Ol(1)Ol(1)ol(2)ol(2)), and a double mutation on both A and B genomes (ol(1)ol(1)ol(2)ol(2)). Each genotype has a significantly different fatty acid profile. Both FAD2A and FAD2B are involved in the conversion of oleic acid to linoleic acid in peanuts. Overall, these results demonstrate the combined power of genetic analysis with biochemical analysis on peanut fatty acid research.
采用气相色谱法对 55 个花生品系进行脂肪酸组成分析，并利用 FAD2 基因的 SNP 标记通过实时 PCR 对其进行基因分型。鉴定出脂肪酸组成存在显著差异，油酸与亚油酸的比例（O/L）范围为 1.23 至 56.45。根据 FAD2 基因突变情况，检测的品系分为四种基因型：野生型（Ol(1)Ol(1)Ol(2)Ol(2)）、A 基因组单个功能纯合突变（ol(1)ol(1)Ol(2)Ol(2)）、B 基因组单个功能纯合突变（Ol(1)Ol(1)ol(2)ol(2)）和 A、B 基因组双突变（ol(1)ol(1)ol(2)ol(2)）。每种基因型的脂肪酸图谱均有显著差异。FAD2A 和 FAD2B 均参与花生中亚油酸向油酸的转化。总的来说，这些结果表明遗传分析与生化分析相结合在花生脂肪酸研究中的强大作用。
