Faculty of Chemistry, Jagiellonian University, Krakow, Poland.
J Agric Food Chem. 2011 Jul 27;59(14):7647-53. doi: 10.1021/jf202366w. Epub 2011 Jun 23.
Food plants from the Apiaceae family have been found to demonstrate health-promoting properties. Polyacetylenes are bioactive compounds that are considered to contribute substantially to the beneficial properties of Apiaceae plants. This study applied a Raman mapping technique in the investigation of polyacetylene spatial distribution in fresh roots of some Apiaceae species. Fresh root sections were measured directly without any preliminary preparation. For three Apiaceae species, that is, parsnip ( Pastinaca sativa L.), celeriac ( Apium graveolens var. rapaceum L.), and parsley ( Petroselinum crispum ), the presence of polyacetylenes was confirmed due to the detection of strong and well-separated Raman signals of symmetric -C ≡ C-C ≡ C- stretching vibration in the range of 2200-2300 cm(-1). The spectra were used for generation of two-dimensional maps applying the integration and cluster analysis methods. The Raman maps visualized the distribution of total polyacetylenes as well as individual compounds. Heterogeneous and tissue-specific occurrence of polyacetylenes in roots is shown.
食用植物的伞形科已被发现具有促进健康的特性。多炔类化合物是具有生物活性的化合物，被认为对伞形科植物的有益特性有重要贡献。本研究应用拉曼映射技术研究了一些伞形科植物新鲜根中多炔的空间分布。直接测量新鲜的根段，无需任何预处理。对于三种伞形科植物，即欧洲防风草（Pastinaca sativa L.）、块根欧芹（Apium graveolens var. rapaceum L.）和欧芹（Petroselinum crispum），由于在 2200-2300 cm（-1）范围内检测到强而分离良好的对称 -C≡C-C≡C-伸缩振动的拉曼信号，证实了多炔的存在。使用这些光谱通过积分和聚类分析方法生成二维图谱。拉曼图谱可视化了总多炔以及单个化合物的分布。显示了根中多炔的不均匀和组织特异性分布。
