Komiya S, Sasaguri Y, Inoue A, Nakashima M, Yamamoto S, Yanagida I, Morimatsu M
Department of Orthopaedic Surgery and Pathology, Faculty of Medicine, Kurume University, Japan.
Clin Orthop Relat Res. 1990 Sep(258):304-9.
Cell culture techniques were used to clarify the histogenesis of giant-cell tumor of bone. Even after passage, nearly all of the mononuclear cells possessed tartrate-resistant acid phosphatase and receptors for eel calcitonin, which are both phenotypic markers for osteoclasts. Eel calcitonin produced an increase in the cyclic adenosine monophosphate (cAMP) content of the mononuclear cells. More than 90% of mononuclear tumor cells expressed monocyte markers; flow cytometric C3b receptor, a macrophage marker, was also detected in a few cells. These findings demonstrate that the mononuclear cells expressed phenotypes of both the osteoclast and monocyte-macrophage and that they originate in a monocyte-macrophage-osteoclast lineage. Giant-cell tumor of bone may thus provide a good model for investigating the mechanism of bone resorption in which cells of osteoclast lineage play a central role.
采用细胞培养技术来阐明骨巨细胞瘤的组织发生。即使传代后，几乎所有单核细胞都具有抗酒石酸酸性磷酸酶和鳗鱼降钙素受体，这两者都是破骨细胞的表型标志物。鳗鱼降钙素使单核细胞的环磷酸腺苷（cAMP）含量增加。超过90%的单核肿瘤细胞表达单核细胞标志物；少数细胞中还检测到流式细胞术C3b受体，这是一种巨噬细胞标志物。这些发现表明，单核细胞表达破骨细胞和单核细胞 - 巨噬细胞的表型，并且它们起源于单核细胞 - 巨噬细胞 - 破骨细胞谱系。因此，骨巨细胞瘤可能为研究破骨细胞谱系细胞起核心作用的骨吸收机制提供一个良好的模型。
