Purification of Epstein-Barr virus associated DNase with affinity chromatography of nasopharyngeal carcinoma patient serum.

Epstein-Barr virus associated DNase in the homogeneous form can be purified by chromatographys using CH-sepharose 4B column conjugated with nasopharyngeal carcinoma patient serum, DNA cellulose and phosphocellulose in that sequence. The molecular weight of this enzyme is shown to be 51 kilo-daltons in silver-staining and immunostains. Among various methods for keeping DNase activity, the addition of BSA and dialysis in glycerol or ethylene glycol immediately after the enzyme purification is suggested.