Chiou J F, Cheng Y C, Hsu M M
Department of Bacteriology, College of Medicine, National Taiwan University, Taipei, ROC.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1990 Feb;23(1):34-43.
Epstein-Barr virus associated DNase in the homogeneous form can be purified by chromatographys using CH-sepharose 4B column conjugated with nasopharyngeal carcinoma patient serum, DNA cellulose and phosphocellulose in that sequence. The molecular weight of this enzyme is shown to be 51 kilo-daltons in silver-staining and immunostains. Among various methods for keeping DNase activity, the addition of BSA and dialysis in glycerol or ethylene glycol immediately after the enzyme purification is suggested.
