Tan R S, Datta A K, Cheng Y C
J Virol. 1982 Dec;44(3):893-9. doi: 10.1128/JVI.44.3.893-899.1982.
The diterpene ester promoter of mouse skin tumors, 12-O-tetradecanoyl-phorbol-13-acetate, induced a DNase activity in the Epstein-Barr virus-producer cell line P3HR-1. The elution patterns of the enzyme from DEAE-cellulose, phosphocellulose, and DNA-cellulose columns were different from virus-associated DNA polymerase activity. The partially purified activity could be neutralized to the extent of 90% by sera of patients with nasopharyngeal carcinoma. Purified immunoglobulin G from sera of nasopharyngeal carcinoma patients inhibited this enzyme and that obtained from superinfected Raji cells to the same extent. The partially purified enzyme preferred native DNA as a substrate over denatured DNA and 3'-terminally labeled activated calf thymus DNA. The activity was inhibited by high ionic strength. Phosphonoformic acid did not have any effect on this enzyme activity.
小鼠皮肤肿瘤的二萜酯启动子，12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯，在爱泼斯坦 - 巴尔病毒产生细胞系P3HR - 1中诱导出一种脱氧核糖核酸酶活性。该酶从二乙氨基乙基纤维素、磷酸纤维素和脱氧核糖核酸纤维素柱上的洗脱模式与病毒相关的脱氧核糖核酸聚合酶活性不同。部分纯化的活性可被鼻咽癌患者的血清中和达90%。从鼻咽癌患者血清中纯化的免疫球蛋白G对该酶以及从超感染的拉吉细胞中获得的酶具有相同程度的抑制作用。部分纯化的酶优先选择天然DNA而非变性DNA和3' - 末端标记的活化小牛胸腺DNA作为底物。该活性受到高离子强度的抑制。膦甲酸对这种酶活性没有任何影响。
