Beinrohr László, Murray-Rust Thomas A, Dyksterhuis Leanne, Závodszky Péter, Gál Péter, Pike Robert N, Wijeyewickrema Lakshmi C
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Hungary.
Methods Enzymol. 2011;499:55-75. doi: 10.1016/B978-0-12-386471-0.00004-3.
C1-inhibitor (serpin G1) is a 105 kDa inhibitor which functions as a major antiinflammatory protein in the body. It has its effects via inhibition of the proteases of the complement system and contact system of coagulation, as well as several direct effects mediated by its unique highly glycosylated N-terminal domain. The serpin controls a number of different proteases very efficiently and for some of these the function is augmented by the cofactor, heparin. Here, we describe the preparation of human plasma and recombinant C1-inhibitor and the basic methods required for their characterization, using the complement enzyme C1s as an example of a target enzyme.
C1抑制剂（丝氨酸蛋白酶抑制剂G1）是一种105 kDa的抑制剂，在体内作为主要的抗炎蛋白发挥作用。它通过抑制补体系统和凝血接触系统的蛋白酶发挥作用，以及通过其独特的高度糖基化的N端结构域介导的一些直接作用。该丝氨酸蛋白酶抑制剂能非常有效地控制多种不同的蛋白酶，对于其中一些蛋白酶，辅因子肝素可增强其功能。在此，我们以补体酶C1s作为靶酶的实例，描述人血浆和重组C1抑制剂的制备及其表征所需的基本方法。
