哺乳动物加帽酶如何读取 CTD 密码子的结构见解。
Structural insights to how mammalian capping enzyme reads the CTD code.
机构信息
Structural Biology Program, Sloan-Kettering Institute, New York, NY 10065, USA.
出版信息
Mol Cell. 2011 Jul 22;43(2):299-310. doi: 10.1016/j.molcel.2011.06.001. Epub 2011 Jun 16.
Abstract
Physical interaction between the phosphorylated RNA polymerase II carboxyl-terminal domain (CTD) and cellular capping enzymes is required for efficient formation of the 5' mRNA cap, the first modification of nascent mRNA. Here, we report the crystal structure of the RNA guanylyltransferase component of mammalian capping enzyme (Mce) bound to a CTD phosphopeptide. The CTD adopts an extended β-like conformation that docks Tyr1 and Ser5-PO(4) onto the Mce nucleotidyltransferase domain. Structure-guided mutational analysis verified that the Mce-CTD interface is a tunable determinant of CTD binding and stimulation of guanylyltransferase activity, and of Mce function in vivo. The location and composition of the CTD binding site on mammalian capping enzyme is distinct from that of a yeast capping enzyme that recognizes the same CTD primary structure. Thus, capping enzymes from different taxa have evolved different strategies to read the CTD code.
摘要
磷酸化 RNA 聚合酶 II C 末端结构域 (CTD) 与细胞加帽酶之间的物理相互作用是有效形成 5' mRNA 帽的必要条件，mRNA 帽是新生 mRNA 的第一个修饰。在这里，我们报告了与 CTD 磷酸肽结合的哺乳动物加帽酶 (Mce) 的 RNA 鸟苷转移酶成分的晶体结构。CTD 采用延伸的 β 样构象，将 Tyr1 和 Ser5-PO(4) 对接至 Mce 核苷酸转移酶结构域。结构导向的突变分析证实，Mce-CTD 界面是 CTD 结合和鸟苷转移酶活性以及 Mce 在体内功能的可调决定因素。哺乳动物加帽酶上的 CTD 结合位点的位置和组成与识别相同 CTD 一级结构的酵母加帽酶不同。因此，来自不同分类群的加帽酶已经进化出不同的策略来读取 CTD 密码。
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本文引用的文献
1
Enzymology of RNA cap synthesis.RNA 帽合成的酶学。
Wiley Interdiscip Rev RNA. 2010 Jul-Aug;1(1):152-72. doi: 10.1002/wrna.19. Epub 2010 May 25.
2
cis-Proline-mediated Ser(P)5 dephosphorylation by the RNA polymerase II C-terminal domain phosphatase Ssu72.顺式脯氨酸介导的 RNA 聚合酶 II C 末端结构域磷酸酶 Ssu72 介导的丝氨酸（P）5 去磷酸化。
J Biol Chem. 2011 Feb 18;286(7):5717-26. doi: 10.1074/jbc.M110.197129. Epub 2010 Dec 15.
3
Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex.人源 symplekin-Ssu72-CTD 磷酸肽复合物的晶体结构。
Nature. 2010 Oct 7;467(7316):729-33. doi: 10.1038/nature09391. Epub 2010 Sep 22.
4
Chemical-genomic dissection of the CTD code.CTD 编码的化学基因组剖析。
Nat Struct Mol Biol. 2010 Sep;17(9):1154-61. doi: 10.1038/nsmb.1900. Epub 2010 Aug 29.
5
Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus.酿酒酵母 Cet1-Ceg1 mRNA 加帽装置的结构。
Structure. 2010 Feb 10;18(2):216-27. doi: 10.1016/j.str.2009.12.009.
6
MolProbity: all-atom structure validation for macromolecular crystallography.MolProbity：用于大分子晶体学的全原子结构验证
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21. doi: 10.1107/S0907444909042073. Epub 2009 Dec 21.
7
Progression through the RNA polymerase II CTD cycle.通过RNA聚合酶II C末端结构域循环的进程。
Mol Cell. 2009 Nov 25;36(4):541-6. doi: 10.1016/j.molcel.2009.10.019.
8
Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7.酵母Rpb1 C末端结构域丝氨酸2、5和7位点的磷酸化。
J Biol Chem. 2009 Sep 25;284(39):26421-6. doi: 10.1074/jbc.M109.028993. Epub 2009 Aug 13.
9
Phaser crystallographic software.相位结晶学软件。
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674. doi: 10.1107/S0021889807021206. Epub 2007 Jul 13.
10
Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the carboxyl-terminal domain of RNA polymerase II.RNA加工因子SCAF8与RNA聚合酶II羧基末端结构域不同磷酸化形式结合的快照。
J Biol Chem. 2008 Aug 15;283(33):22659-69. doi: 10.1074/jbc.M803540200. Epub 2008 Jun 11.
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