抗突变型瓜氨酸化波形蛋白（anti-MCV）和抗 65 kDa 热休克蛋白（anti-hsp65）：强直性脊柱炎的新生物标志物。

Anti-mutated citrullinated vimentin (anti-MCV) and anti-65 kDa heat shock protein (anti-hsp65): new biomarkers in ankylosing spondylitis.

机构信息

Department of rheumatology, institute of medicine, medical and health science center, university of Debrecen, 98, Nagyerdei street, 4012 Debrecen, Hungary.

出版信息

Joint Bone Spine. 2012 Jan;79(1):63-6. doi: 10.1016/j.jbspin.2011.03.010. Epub 2011 Jun 16.

DOI:10.1016/j.jbspin.2011.03.010

PMID:21683641

Abstract

INTRODUCTION

Citrullination as well as anti-citrullinated protein/peptide antibodies (ACPA) have been implicated in the pathogenesis of rheumatoid arthritis (RA). While ACPAs are specific and sensitive markers for RA, there have been hardly any reports regarding ACPAs in ankylosing spondylitis (AS). The possible role of antibodies to Mycobacterial 65 kDa heat shock protein (hsp65) has not been characterized in AS. As new laboratory biomarkers of AS are needed, we investigated the prevalence of anti-mutated citrullinated vimentin (MCV) and anti-hsp65 antibodies in AS.

METHODS

Altogether 43 AS and 44 healthy controls were included in the study. Anti-MCV and anti-hsp65 were determined in sera by commercial and in-house ELISA, respectively. Serum autoantibody levels were correlated with ESR, CRP, HLA-B27 status, smoking habits, pain intensity, BASDAI, BASFI and BASMI indices.

RESULTS

Patients with AS had significantly higher serum anti-MCV levels (17.3 U/mL, range: 8.3-31.5 U/mL) in comparison to healthy subjects (8.9 U/mL, range: 5.4-13.3 U/mL) (p<0.01). Sixteen of the 43 AS patients (37%) and none of the 44 healthy controls (0%) were anti-MCV positive using the cut-off value recommended by the manufacturer (>20 U/mL). The mean anti-hsp65 concentration in AS sera was 124.8 AU/mL (range: 27.2-1000 AU/mL), while controls exerted significantly lower anti-hsp65 levels (mean: 51.8 AU/mL; range: 22.5-88.5 AU/mL) (p<0.001). Correlation analysis revealed that both anti-MCV positivity (r=0.613; p=0.012) and absolute serum anti-MCV levels (r=0.553; p=0.021) correlated with anti-hsp65 levels. Anti-MCV positivity also correlated with ESR (r=0.437; p=0.03).

CONCLUSIONS

Anti-MCV and anti-hsp65 may be novel biomarkers in AS.

摘要

简介

瓜氨酸化以及抗瓜氨酸化蛋白/肽抗体（ACPA）已被认为与类风湿关节炎（RA）的发病机制有关。虽然 ACPA 是 RA 的特异性和敏感性标志物，但在强直性脊柱炎（AS）中几乎没有关于 ACPA 的报道。分枝杆菌 65kDa 热休克蛋白（hsp65）抗体在 AS 中的可能作用尚未确定。由于需要新的 AS 实验室生物标志物，我们研究了 AS 中抗突变的瓜氨酸化波形蛋白（MCV）和抗 hsp65 抗体的患病率。

方法

共纳入 43 例 AS 患者和 44 例健康对照者。通过商业和内部 ELISA 分别检测血清中的抗-MCV 和抗-hsp65。血清自身抗体水平与 ESR、CRP、HLA-B27 状态、吸烟习惯、疼痛强度、BASDAI、BASFI 和 BASMI 指数相关。

结果

与健康对照组（8.9 U/mL，范围：5.4-13.3 U/mL）相比，AS 患者的血清抗-MCV 水平明显升高（17.3 U/mL，范围：8.3-31.5 U/mL）（p<0.01）。使用制造商推荐的临界值（>20 U/mL），43 例 AS 患者中有 16 例（37%）为抗-MCV 阳性，而 44 例健康对照组均为阴性（0%）（p<0.01）。AS 血清中抗-hsp65 的平均浓度为 124.8 AU/mL（范围：27.2-1000 AU/mL），而对照组的抗-hsp65 水平明显较低（平均：51.8 AU/mL；范围：22.5-88.5 AU/mL）（p<0.001）。相关性分析表明，抗-MCV 阳性（r=0.613；p=0.012）和血清抗-MCV 绝对水平（r=0.553；p=0.021）与抗-hsp65 水平相关。抗-MCV 阳性也与 ESR 相关（r=0.437；p=0.03）。