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Forensic Sci Int Genet. 2013 Jan;7(1):1-9. doi: 10.1016/j.fsigen.2011.05.009. Epub 2011 Jun 17.
Mitochondrial DNA is commonly used in identity testing for the analysis of old or degraded samples or to give evidence of familial links. The Abbott T5000 mass spectrometry platform provides an alternative to the more commonly used Sanger sequencing for the analysis of human mitochondrial DNA. The robustness of the T5000 system has previously been demonstrated using DNA extracted from volunteer buccal swabs but the system has not been tested using more challenging sample types. For mass spectrometry to be considered as a valid alternative to Sanger sequencing it must also be demonstrated to be suitable for use with more limiting sample types such as old teeth, bone fragments, and hair shafts. In 2009 the Commonwealth War Graves Commission launched a project to identify the remains of 250 World War I soldiers discovered in a mass grave in Fromelles, France. This study characterises the performance of both Sanger sequencing and the T5000 platform for the analysis of the mitochondrial DNA extracted from 225 of these remains, both in terms of the ability to amplify and characterise DNA regions of interest and the relative information content and ease-of-use associated with each method.
线粒体 DNA 通常用于身份鉴定，用于分析陈旧或降解的样本，或提供家族关系的证据。Abbott T5000 质谱平台为人类线粒体 DNA 的分析提供了比更常用的 Sanger 测序更具替代性的方法。T5000 系统的稳健性之前已通过从志愿者口腔拭子中提取的 DNA 得到证明，但尚未使用更具挑战性的样本类型对其进行测试。要将质谱分析视为 Sanger 测序的有效替代方法，还必须证明它也适用于更有限的样本类型，如陈旧的牙齿、骨碎片和头发。2009 年，英联邦战争坟墓委员会发起了一个项目，以确定在法国弗勒吕斯的一个万人冢中发现的 250 名一战士兵的遗体。本研究对 Sanger 测序和 T5000 平台从这些遗体中的 225 具遗体中提取的线粒体 DNA 的分析性能进行了描述，包括对感兴趣 DNA 区域的扩增和特征分析的能力，以及与每种方法相关的相对信息量和易用性。
