Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
Dev Comp Immunol. 2012 Jan;36(1):230-5. doi: 10.1016/j.dci.2011.05.011. Epub 2011 Jun 12.
Several lectin families characterized by distinct signature sequence motifs and structural folds, such as C-type, peptidoglycan recognition protein, ficolin, pentraxins, and most recently galectins, have been implicated in immune surveillance. In this study, two distinct F-type lectins RbFTL-1 and RbFTL-2, from the rock bream (Oplegnathus fasciatus), were identified and their expression was analyzed. The full-length cDNA of RbFTL-1 was composed of 1204 bp with a 945-bp open reading frame (ORF) that encoded a 314 amino-acid protein, while that of RbFTL-2 consisted of 1614 bp with a 951-bp ORF encoding a 316 amino-acid protein. RbFTL-1 and RbFTL-2 mRNAs were predominately expressed in the head-kidney and in the liver, respectively. Levels of the RbFTL-1 mRNA transcript increased up to 5.0- and 2.8-fold in the head-kidney and trunk-kidney compared to the muscle, respectively, while those of the RbFTL-2 mRNA transcript increased up to 12.0-fold in liver. The expression of RbFTL-1 and RbFTL-2 were differentially up-regulated in rock bream challenged with Edwardsiella tarda, Streptococcus iniae, and RSIV, with significant increases at 1 and 3h post-challenge compared to the controls.
几种凝集素家族以独特的特征序列基序和结构折叠为特征，如 C 型、肽聚糖识别蛋白、ficolin、五聚体和最近的半乳糖凝集素，它们都与免疫监视有关。在这项研究中，从石斑鱼（Oplegnathus fasciatus）中鉴定出两种不同的 F 型凝集素 RbFTL-1 和 RbFTL-2，并分析了它们的表达。RbFTL-1 的全长 cDNA 由 1204bp 组成，其中 945bp 为开放阅读框（ORF），编码 314 个氨基酸的蛋白质，而 RbFTL-2 的全长 cDNA 由 1614bp 组成，其中 951bp ORF 编码 316 个氨基酸的蛋白质。RbFTL-1 和 RbFTL-2 的 mRNA 主要在头部肾脏和肝脏中表达。与肌肉相比，RbFTL-1 的 mRNA 转录水平在头部肾脏和躯干肾脏中分别增加了 5.0-和 2.8 倍，而 RbFTL-2 的 mRNA 转录水平在肝脏中增加了 12.0 倍。RbFTL-1 和 RbFTL-2 的表达在石斑鱼受到迟钝爱德华氏菌、链球菌和传染性造血器官坏死病毒（RSIV）挑战时被差异上调，与对照组相比，在 1 小时和 3 小时后显著增加。
