Park Hyung-Jun, Jeong Ji-Min, Bae Jin-Sol, Kim Ju-Won, An Cheul-Min, Min Byung-Hwa, Kim Seong-Yeon, Myeong Jeong-In, Hwang Hyung-Kyu, Park Chan-Il
Aquaculture Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.
Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 53064, Republic of Korea.
Dev Comp Immunol. 2016 Dec;65:25-30. doi: 10.1016/j.dci.2016.06.014. Epub 2016 Jun 21.
A new lily-type lectin RbLTL was identified from rock bream (Oplegnathus fasciatus) and its expression analysed. In this study, a new lily-type lectin gene (RbLTL) was cloned from rock bream using expressed sequence tag (EST) analysis. The full-length RbLTL cDNA was encoding a 117-amino acid protein. The deduced amino acid sequence of RbLTL contained all of the conserved features crucial for its fundamental structure, including B-lectin domain and three d-mannose binding sites. RbLTL mRNA was predominately expressed in the gills, with reduced expression noted in intestine tissue. Expression analysis of time series sampled fertilized eggs revealed that expression gradually increased 1, 3, 12, and 24 h: However, expression decreased at 36 h. RbLTL expression was differentially up-regulated in rock bream gills challenged with Streptococcus iniae, Edwardsiella tarda and RSIV. Our results revealed that novel rock bream lily-type lectin may be an important molecule involved in pattern recognition and pathogen elimination in the innate immunity of rock bream.
从条石鲷（Oplegnathus fasciatus）中鉴定出一种新的百合型凝集素RbLTL，并对其表达进行了分析。在本研究中，利用表达序列标签（EST）分析从条石鲷中克隆出一个新的百合型凝集素基因（RbLTL）。RbLTL cDNA全长编码一个117个氨基酸的蛋白质。RbLTL推导的氨基酸序列包含对其基本结构至关重要的所有保守特征，包括B-凝集素结构域和三个D-甘露糖结合位点。RbLTL mRNA主要在鳃中表达，在肠道组织中的表达降低。对受精后不同时间点的鱼卵进行的时间序列表达分析显示，在1、3、12和24小时表达逐渐增加，然而在36小时表达下降。在用海豚链球菌、迟缓爱德华氏菌和虹彩病毒感染的条石鲷鳃中，RbLTL表达差异上调。我们的结果表明，条石鲷新的百合型凝集素可能是参与条石鲷先天免疫中模式识别和病原体清除的重要分子。
