Lee Young Mee, Yang In Jung, Noh Jae Koo, Kim Hyun Chul, Park Choul-Ji, Park Jong-Won, Noh Gyeong Eon, Kim Woo-Jin, Kim Kyung-Kil
Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
Dev Reprod. 2016 Dec;20(4):297-304. doi: 10.12717/DR.2016.20.4.297. Epub 2016 Dec 31.
Lectins belong to the pattern-recognition receptors (PRRs) class and play important roles in the recognition and elimination of pathogens via the innate immune system. Recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks in the early immune response of fish. However, this study is limited to information that the lectin is involved in the innate immune response against viral infection. In the present study, the lily-type lectin-2 and -3 of (OfLTL-2 and 3) have been presented to be included B-lectin domain and two D-mannose binding sites in the amino acid sequence that an important feature for the fundamental structure. To investigate the functional properties of OfLTLs, the tissue distribution in the healthy rock bream and temporal expression during early developmental stage analysis are performed using quantitative real-time PCR. OfLTL-2 and 3 are predominantly expressed in the liver and skin, but rarely expressed in other organ. Also, the transcripts of OfLTLs are not expressed during the early developmental stage but its transcripts are increased after immune-related organs which are fully formed. In the challenge experiment with RBIV (rock bream iridovirus), the expression of OfLTLs was increased much more strongly in the late response than the early, unlike previously known. These results suggest that OfLTLs are specifically expressed in the immune-related tissues when those organs are fully formed and it can be inferred that the more intensively involved in the second half to the virus infection.
凝集素属于模式识别受体（PRRs）类别，在通过先天免疫系统识别和清除病原体方面发挥着重要作用。最近，有报道称在病原体攻击鱼类的早期免疫反应中，百合型凝集素-1会参与其中。然而，这项研究仅限于该凝集素参与针对病毒感染的先天免疫反应的信息。在本研究中，已证实 （OfLTL-2 和 3）的百合型凝集素-2 和 -3 在氨基酸序列中包含 B-凝集素结构域和两个 D-甘露糖结合位点，这是其基本结构的一个重要特征。为了研究 OfLTLs 的功能特性，使用定量实时 PCR 分析了健康条石鲷中的组织分布以及早期发育阶段的时间表达情况。OfLTL-2 和 3 主要在肝脏和皮肤中表达，但在其他器官中很少表达。此外，OfLTLs 的转录本在早期发育阶段不表达，但其转录本在免疫相关器官完全形成后增加。在使用条石鲷虹彩病毒（RBIV）进行的攻毒实验中，与之前已知的情况不同，OfLTLs 的表达在后期反应中比早期增加得更为强烈。这些结果表明，OfLTLs 在免疫相关组织完全形成时在其中特异性表达，可以推断其在病毒感染后期更强烈地参与其中。
