Università degli Studi di Padova, Dipartimento di Scienze Farmaceutiche, Via F. Marzolo, 5, 35131 Padova, Italy.
Phytomedicine. 2011 Sep 15;18(12):1031-6. doi: 10.1016/j.phymed.2011.05.005.
We investigated the effects of Achillea millefolium extract in vitro on the growth of primary rat vascular smooth muscle cells (VSMCs) as well as the potential involvement of estrogen receptors (ERs) in this process. In addition, the ability of A. millefolium extract to modulate the NF-κB pathway was tested in human umbilical vein endothelial cells (HUVECs). The fingerprinting of the extract was carried out by HPLC-DAD and LC-MS(n) and main constituents were flavonoids (10%) and dicaffeolylquinic acid derivatives (12%). The extract enhanced VSMC growth at least in part by acting through ERs and impaired NF-κB signaling in HUVECs. The various compounds may act with different mode of actions thus contributing to the final effect of the extract. Our findings support some of the traditional uses of A. millefolium, and suggest potential modes of action as related to its effects on vascular inflammation. Therefore, A. millefolium may induce novel potential actions in the cardiovascular system.
我们研究了蓍草提取物在体外对原代大鼠血管平滑肌细胞（VSMCs）生长的影响，以及雌激素受体（ERs）在这一过程中的潜在作用。此外，还测试了蓍草提取物在人脐静脉内皮细胞（HUVECs）中调节 NF-κB 通路的能力。通过 HPLC-DAD 和 LC-MS(n) 对提取物进行了指纹图谱分析，主要成分为类黄酮（10%）和二咖啡酰奎宁酸衍生物（12%）。该提取物至少部分通过 ERs 作用增强 VSMC 生长，并损害 HUVECs 中的 NF-κB 信号。各种化合物可能通过不同的作用方式发挥作用，从而对提取物的最终效果产生影响。我们的研究结果支持了蓍草的一些传统用途，并提出了与血管炎症相关的潜在作用机制。因此，蓍草可能在心血管系统中引起新的潜在作用。
