Department of Operative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany.
Biochem Biophys Res Commun. 2011 Jul 8;410(3):587-92. doi: 10.1016/j.bbrc.2011.06.031. Epub 2011 Jun 12.
The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs.
干细胞的分化可以通过组织细胞的硬度梯度来引导。例如，坚硬的细胞外基质可以支持骨髓间充质干细胞（MSCs）的成骨分化。然而，关于细胞外基质硬度与外胚层牙齿前体细胞的细胞分化之间的关系，人们知之甚少。我们的研究首次考察了表面硬度对人牙囊细胞（DFCs）增殖和成骨分化的影响。在具有类骨硬度的细胞培养表面上，DFC 的细胞增殖仅略有减少。只有在使用不同硬度的情况下，使用基于地塞米松的分化培养基才能启动 DFCs 的成骨分化。在这里，与最硬的表面相比，最软的表面可更好地诱导成骨分化。总之，与骨髓来源的 MSC 不同，柔软的细胞外基质具有更好的能力来支持 DFC 的成骨分化。
