Morsczeck Christian, Gresser Jan, Ettl Tobias
Department of Oral and Maxillofacial Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
Mol Cell Biochem. 2016 Jun;417(1-2):1-6. doi: 10.1007/s11010-016-2708-z. Epub 2016 May 10.
Dental stem cells such as human dental follicle cells (DFCs) have opened new promising treatment alternatives for today's dental health issues such as periodontal tissue regeneration. However, cellular senescence represents a restricting factor to cultured stem cells, resulting in limited lifespan and reduced cell differentiation potential. Therefore, this study evaluated if and how DFCs exhibit features of cellular senescence after being expanded in cell culture. The cell proliferation of DFCs decreased, while the cell size increased during prolonged cell culture. Moreover, DFCs expressed the senescence-associated β-galactosidase after a prolonged cell culture. The onset of senescence inhibited both the induction of osteoblast markers RUNX2 and osteopontin and the biomineralization of DFCs after stimulation of the osteogenic differentiation. In conclusion, we showed that a prolonged cell culture induces cellular senescence and inhibits the osteogenic differentiation in DFCs.
诸如人牙囊细胞（DFCs）之类的牙干细胞为当今的牙齿健康问题（如牙周组织再生）开辟了新的、有前景的治疗选择。然而，细胞衰老对培养的干细胞来说是一个限制因素，导致其寿命有限且细胞分化潜能降低。因此，本研究评估了DFCs在细胞培养中扩增后是否以及如何表现出细胞衰老的特征。在长时间细胞培养过程中，DFCs的细胞增殖减少，而细胞大小增加。此外，长时间细胞培养后，DFCs表达了衰老相关的β-半乳糖苷酶。衰老的发生抑制了成骨细胞标志物RUNX2和骨桥蛋白的诱导以及DFCs在成骨分化刺激后的生物矿化。总之，我们表明长时间细胞培养会诱导DFCs发生细胞衰老并抑制其成骨分化。
