Shindo K, Matsumoto Y, Hirai Y, Sumitomo M, Amano T, Miyakawa K, Matsumura M, Mizuno T
First Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
J Intern Med. 1990 Aug;228(2):91-6. doi: 10.1111/j.1365-2796.1990.tb00200.x.
To investigate whether leukotriene B4 is present in the arterial blood of asthmatic patients during wheezing attacks, 20 ml of arterial blood was drawn from the inguinal artery of five patients. Leukotriene B4 was detected in all five individuals, and its identity was confirmed by a combination of high pressure liquid chromatography and radioimmunoassay techniques. The concentration of leukotriene B4 was 48.34 +/- 16.27 pg ml-1 (mean value +/- SE). However, in five control subjects the leukotriene B4 concentration was found to be 9.43 +/- 5.44 pg ml-1. Thus there was a significant difference between the two groups (P less than 0.05). These results suggest that leukotriene B4 may be important in elucidation of the pathogenesis of bronchial asthma.
为了研究白三烯B4在哮喘患者喘息发作时的动脉血中是否存在，从5名患者的腹股沟动脉抽取了20毫升动脉血。在所有5名个体中均检测到白三烯B4，其身份通过高压液相色谱和放射免疫分析技术相结合得以确认。白三烯B4的浓度为48.34±16.27皮克/毫升（平均值±标准误）。然而，在5名对照受试者中，发现白三烯B4浓度为9.43±5.44皮克/毫升。因此，两组之间存在显著差异（P<0.05）。这些结果表明，白三烯B4可能在阐明支气管哮喘的发病机制中起重要作用。
