PCR 技术与呼吸道样本中产铜绿假单胞菌的检测：文献综述。
PCR and the detection of Pseudomonas aeruginosa in respiratory samples of CF patients. A literature review.
机构信息
Laboratory Bacteriology Research, University of Ghent, De Pintelaan 185, 9000 Ghent, Belgium.
出版信息
J Cyst Fibros. 2011 Sep;10(5):293-7. doi: 10.1016/j.jcf.2011.05.004.
Previous studies proved the importance of rapid antibacterial intervention in case of Pseudomonas aeruginosa detection in respiratory samples of cystic fibrosis patients. To improve the early detection of P. aeruginosa, several culture, PCR and serology based approaches have been compared. Because an increasing number of routine microbiology laboratories have access to real-time PCR (qPCR), we reviewed the specificity and sensitivity of published PCR formats. The importance of choice of DNA-extraction methods and PCR formats and of the validation of their specificity and sensitivity with clinical samples is stressed.
先前的研究证明了在囊性纤维化患者的呼吸道样本中检测到铜绿假单胞菌时，迅速进行抗菌干预的重要性。为了提高铜绿假单胞菌的早期检测，已经比较了几种基于培养、PCR 和血清学的方法。由于越来越多的常规微生物学实验室可以使用实时 PCR（qPCR），我们回顾了已发表的 PCR 格式的特异性和灵敏度。强调了选择 DNA 提取方法和 PCR 格式的重要性，以及用临床样本验证其特异性和灵敏度的重要性。
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