Wang Hongying, Chi Zhe, Cong Ying, Wang Zhuangzhuang, Jiang Fei, Geng Jiayue, Zhang Peng, Ju Peng, Dong Quanjiang, Liu Chenguang
College of Marine Life Science, Ocean University of China Qingdao 266003 P. R. China
Research Centre of Plant Functional Components, Tobacco Research Institute of Chinese Academy of Agricultural Sciences Qingdao 266001 P. R. China
RSC Adv. 2018 Sep 19;8(57):32454-32460. doi: 10.1039/c8ra04819c. eCollection 2018 Sep 18.
An aptamer-based fluorescence assay for culture-independent detection of was developed. This assay was enabled by highly specific aptamers conjugated with photoluminescent carbon dots (CDs) as the fluorescent probe and graphene oxide (GO) as the quencher. Specially, high-throughput sequencing was achieved during systematic evolution of ligands exponential enrichment (SELEX) for accurate recognition of aptamers. This assay displayed high specificity towards and was resistant to interference by other ubiquitous bacteria including , , , , and . After the conditions were optimized, this assay achieved a wide detection range for varying from 10 CFU mL to 10 CFU mL. Notably, it approached an excellent detection limit as low as 9 CFU mL. Therefore, this fluorescence assay was considered successfully developed for highly sensitive detection of . This assay also detected the contamination of in tap water and commercial bottled water, thereby suggesting its potential application in real water samples.
开发了一种基于适配体的荧光测定法，用于非培养法检测[具体检测对象未给出]。该测定法通过将与光致发光碳点（CDs）偶联的高特异性适配体作为荧光探针，以及氧化石墨烯（GO）作为猝灭剂来实现。特别地，在配体指数富集系统进化（SELEX）过程中实现了高通量测序，以准确识别适配体。该测定法对[具体检测对象未给出]表现出高特异性，并且对包括[其他细菌名称未给出]、[其他细菌名称未给出]、[其他细菌名称未给出]、[其他细菌名称未给出]和[其他细菌名称未给出]在内的其他常见细菌的干扰具有抗性。在条件优化后，该测定法对[具体检测对象未给出]实现了从10 CFU/mL到10 CFU/mL的宽检测范围。值得注意的是，它达到了低至9 CFU/mL的出色检测限。因此，该荧光测定法被认为成功开发用于[具体检测对象未给出]的高灵敏检测。该测定法还检测了自来水和市售瓶装水中[具体检测对象未给出]的污染，从而表明其在实际水样中的潜在应用。
